Matches in SemOpenAlex for { <https://semopenalex.org/work/W4230544029> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W4230544029 abstract "Abstract Background: Gastric cancer is the fourth most common malignant disease. Both CDK10 and long noncoding RNAs (lncRNAs) have been found to exert biological functions in multiple cancers. However, it is still unclear whether CDK10 represses tumor progression in gastric cancer by reducing potential targeting lncRNAs. Methods: The functions of CDK10 and lncRNA-C5ORF42-5 in proliferation, invasion and migration were assessed by MTS assays, colony formation assays, cell cycle and apoptosis assays, Transwell assays, wound healing assays and animal experiments. We used high-throughput sequencing to confirm the existence of lncRNA-C5ORF42-5 and quantitative real-time PCR was used to evaluate lncRNA expression. Then, with RNA-seq sequencing as well as GO function and KEGG enrichment analysis, we identified the signaling pathways in which lncRNA-C5ORF42-5 was involved in gastric cancer. Finally, western blotting was used to identify the genes regulated by lncRNA-C5ORF42-5. Results: Our results showed that CDK10 is expressed at relatively low levels in gastric cancer cell lines and inhibits the progression of gastric cancer cells both in vitro and in vivo. Next, based on high-throughput sequencing, we identified a novel lncRNA, lncRNA-C5ORF42-5, in the stable CDK10-overexpressing cell line compared with the CDK-knockdown cell line and their controls. Additionally, we confirmed that lncRNA-C5ORF42-5 acts as an oncogene to promote metastasis in gastric cancer in vitro and in vivo. We then ascertained that lncRNA-C5ORF42-5 is a major contributor to the function of CDK10 in gastric cancer metastasis by upregulating lncRNA-C5ORF42-5 to reverse the effects of CDK10 overexpression. Finally, we explored the mechanism by which lncRNA-C5ORF42-5 overexpression affects gastric cancer cells to elucidate whether lncRNA-C5ORF42-5 may increase the activity of the SMAD pathway of BMP signaling and promote the expression of EMT-related proteins, such as E-cadherin. Additionally, overexpression of lncRNA-C5ORF42-5 affected the phosphorylation levels of AKT and ERK. Conclusion: Our findings suggest that CDK10 overexpression represses gastric cancer tumor progression by reducing lncRNA-C5ORF42-5 and hindering activation of the related proteins in metastatic signaling pathways, which provides new insight into developing effective therapeutic strategies in the treatment of metastatic gastric cancer." @default.
- W4230544029 created "2022-05-11" @default.
- W4230544029 creator A5027862454 @default.
- W4230544029 creator A5036262283 @default.
- W4230544029 creator A5036273689 @default.
- W4230544029 creator A5064931883 @default.
- W4230544029 creator A5071255900 @default.
- W4230544029 creator A5074310982 @default.
- W4230544029 creator A5087501279 @default.
- W4230544029 date "2021-08-02" @default.
- W4230544029 modified "2023-10-18" @default.
- W4230544029 title "CDK10 Regulates Gastric Cancer Metastasis By Inhibiting lncRNA-C5ORF42-5 Via Phosphorylation Level of AKT/ERK" @default.
- W4230544029 doi "https://doi.org/10.21203/rs.3.rs-610700/v2" @default.
- W4230544029 hasPublicationYear "2021" @default.
- W4230544029 type Work @default.
- W4230544029 citedByCount "0" @default.
- W4230544029 crossrefType "posted-content" @default.
- W4230544029 hasAuthorship W4230544029A5027862454 @default.
- W4230544029 hasAuthorship W4230544029A5036262283 @default.
- W4230544029 hasAuthorship W4230544029A5036273689 @default.
- W4230544029 hasAuthorship W4230544029A5064931883 @default.
- W4230544029 hasAuthorship W4230544029A5071255900 @default.
- W4230544029 hasAuthorship W4230544029A5074310982 @default.
- W4230544029 hasAuthorship W4230544029A5087501279 @default.
- W4230544029 hasBestOaLocation W42305440291 @default.
- W4230544029 hasConcept C104317684 @default.
- W4230544029 hasConcept C121608353 @default.
- W4230544029 hasConcept C173396325 @default.
- W4230544029 hasConcept C2779013556 @default.
- W4230544029 hasConcept C2781018059 @default.
- W4230544029 hasConcept C29537977 @default.
- W4230544029 hasConcept C502942594 @default.
- W4230544029 hasConcept C54355233 @default.
- W4230544029 hasConcept C57074206 @default.
- W4230544029 hasConcept C62112901 @default.
- W4230544029 hasConcept C62203573 @default.
- W4230544029 hasConcept C62478195 @default.
- W4230544029 hasConcept C67705224 @default.
- W4230544029 hasConcept C81885089 @default.
- W4230544029 hasConcept C86803240 @default.
- W4230544029 hasConcept C95444343 @default.
- W4230544029 hasConcept C96232424 @default.
- W4230544029 hasConceptScore W4230544029C104317684 @default.
- W4230544029 hasConceptScore W4230544029C121608353 @default.
- W4230544029 hasConceptScore W4230544029C173396325 @default.
- W4230544029 hasConceptScore W4230544029C2779013556 @default.
- W4230544029 hasConceptScore W4230544029C2781018059 @default.
- W4230544029 hasConceptScore W4230544029C29537977 @default.
- W4230544029 hasConceptScore W4230544029C502942594 @default.
- W4230544029 hasConceptScore W4230544029C54355233 @default.
- W4230544029 hasConceptScore W4230544029C57074206 @default.
- W4230544029 hasConceptScore W4230544029C62112901 @default.
- W4230544029 hasConceptScore W4230544029C62203573 @default.
- W4230544029 hasConceptScore W4230544029C62478195 @default.
- W4230544029 hasConceptScore W4230544029C67705224 @default.
- W4230544029 hasConceptScore W4230544029C81885089 @default.
- W4230544029 hasConceptScore W4230544029C86803240 @default.
- W4230544029 hasConceptScore W4230544029C95444343 @default.
- W4230544029 hasConceptScore W4230544029C96232424 @default.
- W4230544029 hasLocation W42305440291 @default.
- W4230544029 hasLocation W42305440292 @default.
- W4230544029 hasOpenAccess W4230544029 @default.
- W4230544029 hasPrimaryLocation W42305440291 @default.
- W4230544029 hasRelatedWork W1887054737 @default.
- W4230544029 hasRelatedWork W2586385055 @default.
- W4230544029 hasRelatedWork W2613842309 @default.
- W4230544029 hasRelatedWork W2797352271 @default.
- W4230544029 hasRelatedWork W2946891580 @default.
- W4230544029 hasRelatedWork W2977485281 @default.
- W4230544029 hasRelatedWork W3092279631 @default.
- W4230544029 hasRelatedWork W3113198240 @default.
- W4230544029 hasRelatedWork W3198179018 @default.
- W4230544029 hasRelatedWork W3199110160 @default.
- W4230544029 isParatext "false" @default.
- W4230544029 isRetracted "false" @default.
- W4230544029 workType "article" @default.