Matches in SemOpenAlex for { <https://semopenalex.org/work/W4230854558> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W4230854558 abstract "Abstract Background: OP(Osteoporosis) is a common bone metabolic disorder in the elderly characterized by loss of bone mass and a tendency to fracture. The mammalian target of rapamycin (mTOR) pathway in autophagy plays an indispensable role in maintaining the stability of the intracellular environment and ensuring the normal physiological functions of cells. Methods: In this study, different concentrations(20, 40, 60, 80, 100, 120, 140, 160, 180 and 200nM) of rapamycin were used to act on MC3T3-E1 osteoblasts for different time lengths(6, 12, 24, 36 and 48 hours). CCK8 was used to detect the proliferative activity of cells and screen suitable rapamycin concentration for subsequent experiments. Western blot and real-time quantitative PCR were used to detect the expression changes of phosphorylated mTOR, upstream and downstream mTOR pathway, autophagy and osteogenic differentiation markers. The expression of LC3 was observed by immunofluorescence. The differentiation ability of osteoblasts was observed by alizarin red and alkaline phosphatase staining. Results: The results showed that the induction of proliferation activity of osteoblasts from 20 nM to 200 nM presented a parabolic feature. After the action time of 50 μM rapamycin exceeded 12 hours, the proportion of S stage cells was significantly increased. The results of gene and protein analysis showed that rapamycin significantly inhibited the phosphorylation of mTOR, and the phosphorylation of the downstream factors of mTOR, 4E-BP1(eIF4E-binding protein 1) and S6K1(p70 ribosomal S6 kinase 1) also decreased. Rapamycin significantly increased the expression of LC3 II (microtubule associated protein 1 light chain 3-α), significantly increased the ratio of LC3II/LC3I, and significantly decreased the expression of p62(sequestosome-1). Rapamycin significantly induced the expression of ALP(Alkaline phosphatase), Runx2(Runt-related transcription factor 2) and osterix. Conclusions: This study confirmed that rapamycin stimulates the autophagy of osteoblasts by inhibiting mTOR and promotes their proliferation and differentiation, suggesting that mTOR may be a potential therapeutic target for osteoporosis." @default.
- W4230854558 created "2022-05-11" @default.
- W4230854558 creator A5016131030 @default.
- W4230854558 creator A5026861174 @default.
- W4230854558 creator A5064311732 @default.
- W4230854558 creator A5065900474 @default.
- W4230854558 creator A5088847089 @default.
- W4230854558 date "2020-06-29" @default.
- W4230854558 modified "2023-10-16" @default.
- W4230854558 title "Inhibition of the Mammalian Target of Rapamycin Pathway Stimulates Osteoblast Proliferation and Differentiation Through Autophagy in MC3T3-E1 Cells" @default.
- W4230854558 doi "https://doi.org/10.21203/rs.3.rs-36977/v1" @default.
- W4230854558 hasPublicationYear "2020" @default.
- W4230854558 type Work @default.
- W4230854558 citedByCount "0" @default.
- W4230854558 crossrefType "posted-content" @default.
- W4230854558 hasAuthorship W4230854558A5016131030 @default.
- W4230854558 hasAuthorship W4230854558A5026861174 @default.
- W4230854558 hasAuthorship W4230854558A5064311732 @default.
- W4230854558 hasAuthorship W4230854558A5065900474 @default.
- W4230854558 hasAuthorship W4230854558A5088847089 @default.
- W4230854558 hasBestOaLocation W42308545581 @default.
- W4230854558 hasConcept C104202773 @default.
- W4230854558 hasConcept C104317684 @default.
- W4230854558 hasConcept C11960822 @default.
- W4230854558 hasConcept C184235292 @default.
- W4230854558 hasConcept C185592680 @default.
- W4230854558 hasConcept C190283241 @default.
- W4230854558 hasConcept C202751555 @default.
- W4230854558 hasConcept C203522944 @default.
- W4230854558 hasConcept C2776415932 @default.
- W4230854558 hasConcept C2778260815 @default.
- W4230854558 hasConcept C2780926556 @default.
- W4230854558 hasConcept C43060935 @default.
- W4230854558 hasConcept C55493867 @default.
- W4230854558 hasConcept C62478195 @default.
- W4230854558 hasConcept C86554907 @default.
- W4230854558 hasConcept C86803240 @default.
- W4230854558 hasConcept C95444343 @default.
- W4230854558 hasConceptScore W4230854558C104202773 @default.
- W4230854558 hasConceptScore W4230854558C104317684 @default.
- W4230854558 hasConceptScore W4230854558C11960822 @default.
- W4230854558 hasConceptScore W4230854558C184235292 @default.
- W4230854558 hasConceptScore W4230854558C185592680 @default.
- W4230854558 hasConceptScore W4230854558C190283241 @default.
- W4230854558 hasConceptScore W4230854558C202751555 @default.
- W4230854558 hasConceptScore W4230854558C203522944 @default.
- W4230854558 hasConceptScore W4230854558C2776415932 @default.
- W4230854558 hasConceptScore W4230854558C2778260815 @default.
- W4230854558 hasConceptScore W4230854558C2780926556 @default.
- W4230854558 hasConceptScore W4230854558C43060935 @default.
- W4230854558 hasConceptScore W4230854558C55493867 @default.
- W4230854558 hasConceptScore W4230854558C62478195 @default.
- W4230854558 hasConceptScore W4230854558C86554907 @default.
- W4230854558 hasConceptScore W4230854558C86803240 @default.
- W4230854558 hasConceptScore W4230854558C95444343 @default.
- W4230854558 hasLocation W42308545581 @default.
- W4230854558 hasOpenAccess W4230854558 @default.
- W4230854558 hasPrimaryLocation W42308545581 @default.
- W4230854558 hasRelatedWork W11112870 @default.
- W4230854558 hasRelatedWork W13169053 @default.
- W4230854558 hasRelatedWork W19879219 @default.
- W4230854558 hasRelatedWork W20501758 @default.
- W4230854558 hasRelatedWork W20748046 @default.
- W4230854558 hasRelatedWork W27997713 @default.
- W4230854558 hasRelatedWork W30393824 @default.
- W4230854558 hasRelatedWork W6428911 @default.
- W4230854558 hasRelatedWork W21205034 @default.
- W4230854558 hasRelatedWork W21318631 @default.
- W4230854558 isParatext "false" @default.
- W4230854558 isRetracted "false" @default.
- W4230854558 workType "article" @default.