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- W4231285815 abstract "Abstract The HEN1 RNA 2′‐ O ‐methyltransferase plays important roles in the biogenesis of small non‐coding RNAs in plants and proved a valuable tool for selective transfer of functional groups from cofactor analogues onto miRNA and siRNA duplexes in vitro. Herein, we demonstrate the versatile HEN1‐mediated methylation and alkylation of small RNA strands in heteroduplexes with a range of complementary synthetic DNA oligonucleotides carrying user‐defined moieties such as internal or 3′‐terminal extensions or chemical reporter groups. The observed DNA‐guided covalent functionalization of RNA broadens our understanding of the substrate specificity of HEN1 and paves the way for the development of novel chemo‐enzymatic tools with potential applications in miRNomics, synthetic biology, and nanomedicine." @default.
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- W4231285815 date "2017-05-04" @default.
- W4231285815 modified "2023-10-17" @default.
- W4231285815 title "Oligonucleotide-Addressed Covalent 3′-Terminal Derivatization of Small RNA Strands for Enrichment and Visualization" @default.
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- W4231285815 doi "https://doi.org/10.1002/ange.201701448" @default.
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