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- W4231520673 abstract "We marked a large number of yeast telomeres within their Y' regions by transforming strains with a fragment of Y' DNA into which the URA3 gene had been inserted. A few of the Ura+ transformants obtained were very unstable and were found to contain autonomously replicating URA3-marked circular Y' elements in high copy number. These marked extrachromosomal circles were capable of reintegrating into the chromosome at other telomeric locations. In contrast, most of the Ura+ transformants obtained were quite stable mitotically and were marked at bona fide chromosomal ends. These stable transformants gave rise to mitotically unstable URA3-marked circular Y' elements at a low frequency (up to 2.5%). The likelihood that such excisions and integrations represent a natural process in Saccharomyces cerevisiae is supported by our identification of putative Y' circles in untransformed strains. The transfer of Y' information among telomeres via a circular intermediate may be important for homogenizing the sequences at the ends of yeast chromosomes and for generating the frequent telomeric rearrangements that have been observed in S. cerevisiae." @default.
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- W4231520673 date "1985-09-01" @default.
- W4231520673 modified "2023-09-26" @default.
- W4231520673 title "Identification of Autonomously Replicating Circular Subtelomeric Y' Elements in <i>Saccharomyces cerevisiae</i>" @default.
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- W4231520673 doi "https://doi.org/10.1128/mcb.5.9.2369-2380.1985" @default.
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