Matches in SemOpenAlex for { <https://semopenalex.org/work/W4231588639> ?p ?o ?g. }
- W4231588639 endingPage "n/a" @default.
- W4231588639 startingPage "n/a" @default.
- W4231588639 abstract "Proliferative scarring is a human disease with neither available effective treatment nor relevant animal model. One of the hypotheses for scar formation involves deregulation of fibroblast signaling and delayed apoptosis. Here, we introduce a new chemical-free method for fibroblast density control in culture by intermittently delivered pulsed electric fields (IDPEF), which cause irreversible damage to cell membranes. Using 5–100 pulses with electric field strength of 150 V/mm, pulse duration 70 µs, and frequency of 1 Hz, we investigated the effects of PEF application on growth, death, and regeneration of normal human dermal fibroblasts in culture. We found that the fraction of fibroblasts that survive depends on the number of pulses applied and follows a Weibull distribution. We have successfully developed an IDPEF protocol that controls fibroblasts density in culture. Specifically, through application of IDPEF every 72 h for 12 days, we maintain a normal human dermal fibroblast density in the 3.1 ± 0.2 × 105–1.4 ± 0.2 × 105 cell/mL range. Our results suggest that IDPEFs may prove useful as a non-chemical method for fibroblast density control in human wound healing. Biotechnol. Bioeng. 2013; 110: 1759–1768. © 2013 Wiley Periodicals, Inc." @default.
- W4231588639 created "2022-05-12" @default.
- W4231588639 creator A5033300219 @default.
- W4231588639 creator A5054618559 @default.
- W4231588639 creator A5055658784 @default.
- W4231588639 creator A5091461486 @default.
- W4231588639 date "2013-01-01" @default.
- W4231588639 modified "2023-09-27" @default.
- W4231588639 title "Regeneration and control of human fibroblast cell density by intermittently delivered pulsed electric fields" @default.
- W4231588639 cites W1513175590 @default.
- W4231588639 cites W1521859708 @default.
- W4231588639 cites W1539442102 @default.
- W4231588639 cites W1965345137 @default.
- W4231588639 cites W1973142530 @default.
- W4231588639 cites W1974185118 @default.
- W4231588639 cites W1976289701 @default.
- W4231588639 cites W1981803595 @default.
- W4231588639 cites W1989963286 @default.
- W4231588639 cites W1995421159 @default.
- W4231588639 cites W2004041110 @default.
- W4231588639 cites W2010461190 @default.
- W4231588639 cites W2010819089 @default.
- W4231588639 cites W2014797299 @default.
- W4231588639 cites W2019068717 @default.
- W4231588639 cites W2021792988 @default.
- W4231588639 cites W2025032647 @default.
- W4231588639 cites W2027256333 @default.
- W4231588639 cites W2029812891 @default.
- W4231588639 cites W2034591737 @default.
- W4231588639 cites W2039942347 @default.
- W4231588639 cites W2061643110 @default.
- W4231588639 cites W2068061989 @default.
- W4231588639 cites W2068647128 @default.
- W4231588639 cites W2080657082 @default.
- W4231588639 cites W2090008802 @default.
- W4231588639 cites W2097165427 @default.
- W4231588639 cites W2104463355 @default.
- W4231588639 cites W2118789657 @default.
- W4231588639 cites W2122808924 @default.
- W4231588639 cites W2126306109 @default.
- W4231588639 cites W2129677951 @default.
- W4231588639 cites W2130183681 @default.
- W4231588639 cites W2140332412 @default.
- W4231588639 cites W2142605112 @default.
- W4231588639 cites W2159092745 @default.
- W4231588639 cites W2325727928 @default.
- W4231588639 cites W2335187016 @default.
- W4231588639 cites W2911320856 @default.
- W4231588639 cites W4239753913 @default.
- W4231588639 cites W4249532770 @default.
- W4231588639 cites W4250362363 @default.
- W4231588639 cites W615066129 @default.
- W4231588639 doi "https://doi.org/10.1002/bit.24832" @default.
- W4231588639 hasPublicationYear "2013" @default.
- W4231588639 type Work @default.
- W4231588639 citedByCount "1" @default.
- W4231588639 countsByYear W42315886392013 @default.
- W4231588639 crossrefType "journal-article" @default.
- W4231588639 hasAuthorship W4231588639A5033300219 @default.
- W4231588639 hasAuthorship W4231588639A5054618559 @default.
- W4231588639 hasAuthorship W4231588639A5055658784 @default.
- W4231588639 hasAuthorship W4231588639A5091461486 @default.
- W4231588639 hasConcept C121332964 @default.
- W4231588639 hasConcept C12554922 @default.
- W4231588639 hasConcept C136229726 @default.
- W4231588639 hasConcept C1491633281 @default.
- W4231588639 hasConcept C171056886 @default.
- W4231588639 hasConcept C185592680 @default.
- W4231588639 hasConcept C190283241 @default.
- W4231588639 hasConcept C202751555 @default.
- W4231588639 hasConcept C203014093 @default.
- W4231588639 hasConcept C2778005187 @default.
- W4231588639 hasConcept C2780269544 @default.
- W4231588639 hasConcept C2780381497 @default.
- W4231588639 hasConcept C55493867 @default.
- W4231588639 hasConcept C60799052 @default.
- W4231588639 hasConcept C62112901 @default.
- W4231588639 hasConcept C62520636 @default.
- W4231588639 hasConcept C71924100 @default.
- W4231588639 hasConcept C86803240 @default.
- W4231588639 hasConcept C95444343 @default.
- W4231588639 hasConceptScore W4231588639C121332964 @default.
- W4231588639 hasConceptScore W4231588639C12554922 @default.
- W4231588639 hasConceptScore W4231588639C136229726 @default.
- W4231588639 hasConceptScore W4231588639C1491633281 @default.
- W4231588639 hasConceptScore W4231588639C171056886 @default.
- W4231588639 hasConceptScore W4231588639C185592680 @default.
- W4231588639 hasConceptScore W4231588639C190283241 @default.
- W4231588639 hasConceptScore W4231588639C202751555 @default.
- W4231588639 hasConceptScore W4231588639C203014093 @default.
- W4231588639 hasConceptScore W4231588639C2778005187 @default.
- W4231588639 hasConceptScore W4231588639C2780269544 @default.
- W4231588639 hasConceptScore W4231588639C2780381497 @default.
- W4231588639 hasConceptScore W4231588639C55493867 @default.
- W4231588639 hasConceptScore W4231588639C60799052 @default.
- W4231588639 hasConceptScore W4231588639C62112901 @default.
- W4231588639 hasConceptScore W4231588639C62520636 @default.
- W4231588639 hasConceptScore W4231588639C71924100 @default.