Matches in SemOpenAlex for { <https://semopenalex.org/work/W4231642427> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W4231642427 abstract "Abstract Background To investigate the potential role of CFTR in myocardial ischemic/reperfusion (I/R) injury and its relationship with mitophagy. Methods Wild type (WT) and age matched CFTR −/− male mice were used to establish the myocardial I/R model. CFTR activator forskolin (FSK) was used to activate CFTR in mice. Hypoxia/reoxygenation (H/R) treatment was used for in vitro model in WT or CFTR −/− cardiomyocytes. The autophagy inhibitor 3-MA and activator rapamycin was used for inhibition or activation of autophagy, respectively. The mitochondrial membrane potential (MMP) and ATP concentration were detected. Immunofluorescence was performed for measurement of mitochondria. Oxidative factors reactive oxygen species (ROS), superoxide dismutase (SOD), malondiadehycle (MDA) and glutathione peroxidase (GSH-PX) were detected. The expression of CFTR, MMP-9, TNF-α, IL-8, LC3 II/I, beclin1, caspase-3, caspase-8, caspase-9, bax, bcl-2, p-62 and FUNDC1 was determined using western blotting or PCR. Results Knockdown of CFTR significantly increased the infraction volume and decreased the expression of autophagy related proteins beclin1 and LC3II/I in mice. In H/R cardiomyocytes, deficiency of CFTR by induced dysfunction of mitochondrial, decrease of ATP concentration and enhanced oxidative stress, as well as inhibited mitophagy and increased cell apoptosis related protein levels. When treated with 3-MA, the effects of overexpression of CFTR was remarkably reversed, while treatment of rapamycin significantly reversed the effects of inhibiting CFTR on both mitophagy, oxidative stress and cell apoptosis related proteins. The inhibition of FUNDC1 also reversed the above effects of overexpressing CFTR. Conclusion Inhibition of CFTR could promote myocardial I/R injury by suppressing FUNDC1-mediated mitophagy and activating of oxidative stress." @default.
- W4231642427 created "2022-05-12" @default.
- W4231642427 creator A5013718214 @default.
- W4231642427 creator A5018422537 @default.
- W4231642427 creator A5026487071 @default.
- W4231642427 creator A5030775427 @default.
- W4231642427 creator A5061015594 @default.
- W4231642427 creator A5074162944 @default.
- W4231642427 creator A5074461919 @default.
- W4231642427 creator A5089488563 @default.
- W4231642427 date "2020-05-04" @default.
- W4231642427 modified "2023-10-16" @default.
- W4231642427 title "CFTR improves myocardial ischemic/reperfusion injury through activating FUNDC1-mediated mitophagy and regulating ATP and mitochondrial functions" @default.
- W4231642427 doi "https://doi.org/10.21203/rs.3.rs-21026/v1" @default.
- W4231642427 hasPublicationYear "2020" @default.
- W4231642427 type Work @default.
- W4231642427 citedByCount "0" @default.
- W4231642427 crossrefType "posted-content" @default.
- W4231642427 hasAuthorship W4231642427A5013718214 @default.
- W4231642427 hasAuthorship W4231642427A5018422537 @default.
- W4231642427 hasAuthorship W4231642427A5026487071 @default.
- W4231642427 hasAuthorship W4231642427A5030775427 @default.
- W4231642427 hasAuthorship W4231642427A5061015594 @default.
- W4231642427 hasAuthorship W4231642427A5074162944 @default.
- W4231642427 hasAuthorship W4231642427A5074461919 @default.
- W4231642427 hasAuthorship W4231642427A5089488563 @default.
- W4231642427 hasBestOaLocation W42316424271 @default.
- W4231642427 hasConcept C126322002 @default.
- W4231642427 hasConcept C134018914 @default.
- W4231642427 hasConcept C153911025 @default.
- W4231642427 hasConcept C170493617 @default.
- W4231642427 hasConcept C185592680 @default.
- W4231642427 hasConcept C190283241 @default.
- W4231642427 hasConcept C203522944 @default.
- W4231642427 hasConcept C2775838275 @default.
- W4231642427 hasConcept C2776151105 @default.
- W4231642427 hasConcept C2778175917 @default.
- W4231642427 hasConcept C2779324830 @default.
- W4231642427 hasConcept C28859421 @default.
- W4231642427 hasConcept C48349386 @default.
- W4231642427 hasConcept C55493867 @default.
- W4231642427 hasConcept C71924100 @default.
- W4231642427 hasConcept C86803240 @default.
- W4231642427 hasConcept C88045685 @default.
- W4231642427 hasConcept C95444343 @default.
- W4231642427 hasConceptScore W4231642427C126322002 @default.
- W4231642427 hasConceptScore W4231642427C134018914 @default.
- W4231642427 hasConceptScore W4231642427C153911025 @default.
- W4231642427 hasConceptScore W4231642427C170493617 @default.
- W4231642427 hasConceptScore W4231642427C185592680 @default.
- W4231642427 hasConceptScore W4231642427C190283241 @default.
- W4231642427 hasConceptScore W4231642427C203522944 @default.
- W4231642427 hasConceptScore W4231642427C2775838275 @default.
- W4231642427 hasConceptScore W4231642427C2776151105 @default.
- W4231642427 hasConceptScore W4231642427C2778175917 @default.
- W4231642427 hasConceptScore W4231642427C2779324830 @default.
- W4231642427 hasConceptScore W4231642427C28859421 @default.
- W4231642427 hasConceptScore W4231642427C48349386 @default.
- W4231642427 hasConceptScore W4231642427C55493867 @default.
- W4231642427 hasConceptScore W4231642427C71924100 @default.
- W4231642427 hasConceptScore W4231642427C86803240 @default.
- W4231642427 hasConceptScore W4231642427C88045685 @default.
- W4231642427 hasConceptScore W4231642427C95444343 @default.
- W4231642427 hasLocation W42316424271 @default.
- W4231642427 hasLocation W42316424272 @default.
- W4231642427 hasOpenAccess W4231642427 @default.
- W4231642427 hasPrimaryLocation W42316424271 @default.
- W4231642427 hasRelatedWork W1967888239 @default.
- W4231642427 hasRelatedWork W1977930150 @default.
- W4231642427 hasRelatedWork W1985538633 @default.
- W4231642427 hasRelatedWork W2016468663 @default.
- W4231642427 hasRelatedWork W2031601225 @default.
- W4231642427 hasRelatedWork W2035242519 @default.
- W4231642427 hasRelatedWork W2904914030 @default.
- W4231642427 hasRelatedWork W3156833871 @default.
- W4231642427 hasRelatedWork W3194998566 @default.
- W4231642427 hasRelatedWork W4320728497 @default.
- W4231642427 isParatext "false" @default.
- W4231642427 isRetracted "false" @default.
- W4231642427 workType "article" @default.