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- W42327767 abstract "Plasmids play important roles in the lives of organisms that have them and have proved invaluable to microbiologists and molecular geneticists in constructs and transferring new genetic combinations cloning gene. Not all organisms and lactic acid bacteria have plasmids. They confer a selective and important advantage on the organisms that possess them such as L. plantarum. Plasmid extraction and electrophoresis of the DNA was carried out on 0.8% agarose gel in a 0.5X concentration of Tris-Borate- EDTA (TBE) buffer. Plasmid DNA bands were viewed by fluorescence of bound ethidium bromide under a short wave ultraviolet light trans-illuminator and the photographs taken. Plasmids curing were done by the use of acridine orange after the growth of the organisms. Physiological studies were done by monitoring the growth through increased turbidity of the isolates at different environmental conditions spectrophotometrically. Forty L. plantarum were screened, only eleven of them possess plasmids; Out of which only three were chosen for further work based on the abundant production of plasmids. These were subjected to different conditions with the cured and uncured isolates. Plasmid curing showed a significant negative effect on growth, physiological characteristics and colony morphology of the L. plantarum." @default.
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- W42327767 date "2015-01-01" @default.
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- W42327767 title "Plasmid Curing and Its Effect on the Growth and Physiological Characteristics of Lactobacillus plantarum Isolated from Fermented Cereals" @default.
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