Matches in SemOpenAlex for { <https://semopenalex.org/work/W4232821856> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W4232821856 abstract "Abstract Background In molecular imaging with dynamic PET, the binding and dissociation of a targeted tracer is characterized by kinetics modeling which requires the arterial concentration of the tracer to be measured accurately. Once in the body the radiolabeled parent tracer may be subjected to hydrolysis, demethylation/dealkylation and other biochemical processes, resulting in the production and accumulation of different metabolites in blood which can be labeled with the same PET radionuclide as the parent. Since these radio-metabolites cannot be distinguished by PET scanning from the parent tracer, their contribution to the arterial concentration curve has to be removed for the accurate estimation of kinetic parameters from kinetic analysis of dynamic PET. High performance liquid chromatography has been used to separate and measure radio-metabolites in blood plasma, however, the method is labor intensive and remains a challenge to implement for each individual patient. The purpose of this study is to develop an alternate technique based on thin layer chromatography (TLC) and a sensitive commercial autoradiography system (Beaver, Ai4R, Nantes, France) to measure radio-metabolites in blood plasma of two targeted tracers - [ 18 F]FAZA and [ 18 F]FEPPA , for imaging hypoxia and inflammation respectively. Results Radioactivity as low as 17Bq in 2µL of pig’s plasma can be detected on the TLC plate using autoradiography. Peaks corresponding to the parent tracer and radio-metabolites could be distinguished in the line profile through each sample (n=8) in the autoradiographic image. Significant inter-subject and intra-subject variability in radio-metabolites production could be observed with both tracers. For [ 18 F]FEPPA, 50% of plasma activity was from radio-metabolites as early as 5 min post injection while for [ 18 F]FAZA, significant metabolites did not appear until 50 min post. Simulation study investigating the effect of radio-metabolite in the estimation of kinetic parameters indicated that 32-400% parameter error can result without radio-metabolites correction. Conclusion TLC coupled with autoradiography is a good alternative to high performance liquid chromatography for radio-metabolite correction. The advantages of requiring only small blood samples (~ 100 μL) and of analyzing multiple samples simultaneously, make the method suitable for individual dynamic PET studies." @default.
- W4232821856 created "2022-05-12" @default.
- W4232821856 creator A5009274582 @default.
- W4232821856 creator A5013499153 @default.
- W4232821856 creator A5060397295 @default.
- W4232821856 creator A5067114921 @default.
- W4232821856 creator A5073210341 @default.
- W4232821856 creator A5073772845 @default.
- W4232821856 creator A5086534403 @default.
- W4232821856 date "2020-09-11" @default.
- W4232821856 modified "2023-09-25" @default.
- W4232821856 title "Plasma radio–metabolite analysis of PET tracers for dynamic PET imaging: TLC and autoradiography" @default.
- W4232821856 doi "https://doi.org/10.21203/rs.3.rs-35592/v3" @default.
- W4232821856 hasPublicationYear "2020" @default.
- W4232821856 type Work @default.
- W4232821856 citedByCount "0" @default.
- W4232821856 crossrefType "posted-content" @default.
- W4232821856 hasAuthorship W4232821856A5009274582 @default.
- W4232821856 hasAuthorship W4232821856A5013499153 @default.
- W4232821856 hasAuthorship W4232821856A5060397295 @default.
- W4232821856 hasAuthorship W4232821856A5067114921 @default.
- W4232821856 hasAuthorship W4232821856A5073210341 @default.
- W4232821856 hasAuthorship W4232821856A5073772845 @default.
- W4232821856 hasAuthorship W4232821856A5086534403 @default.
- W4232821856 hasBestOaLocation W42328218561 @default.
- W4232821856 hasConcept C104317684 @default.
- W4232821856 hasConcept C121332964 @default.
- W4232821856 hasConcept C150194340 @default.
- W4232821856 hasConcept C185544564 @default.
- W4232821856 hasConcept C185592680 @default.
- W4232821856 hasConcept C190196608 @default.
- W4232821856 hasConcept C190727270 @default.
- W4232821856 hasConcept C202199734 @default.
- W4232821856 hasConcept C2775842073 @default.
- W4232821856 hasConcept C2777477808 @default.
- W4232821856 hasConcept C2778863792 @default.
- W4232821856 hasConcept C2989005 @default.
- W4232821856 hasConcept C3017724952 @default.
- W4232821856 hasConcept C43617362 @default.
- W4232821856 hasConcept C55493867 @default.
- W4232821856 hasConcept C71924100 @default.
- W4232821856 hasConceptScore W4232821856C104317684 @default.
- W4232821856 hasConceptScore W4232821856C121332964 @default.
- W4232821856 hasConceptScore W4232821856C150194340 @default.
- W4232821856 hasConceptScore W4232821856C185544564 @default.
- W4232821856 hasConceptScore W4232821856C185592680 @default.
- W4232821856 hasConceptScore W4232821856C190196608 @default.
- W4232821856 hasConceptScore W4232821856C190727270 @default.
- W4232821856 hasConceptScore W4232821856C202199734 @default.
- W4232821856 hasConceptScore W4232821856C2775842073 @default.
- W4232821856 hasConceptScore W4232821856C2777477808 @default.
- W4232821856 hasConceptScore W4232821856C2778863792 @default.
- W4232821856 hasConceptScore W4232821856C2989005 @default.
- W4232821856 hasConceptScore W4232821856C3017724952 @default.
- W4232821856 hasConceptScore W4232821856C43617362 @default.
- W4232821856 hasConceptScore W4232821856C55493867 @default.
- W4232821856 hasConceptScore W4232821856C71924100 @default.
- W4232821856 hasLocation W42328218561 @default.
- W4232821856 hasLocation W42328218562 @default.
- W4232821856 hasOpenAccess W4232821856 @default.
- W4232821856 hasPrimaryLocation W42328218561 @default.
- W4232821856 hasRelatedWork W1978393454 @default.
- W4232821856 hasRelatedWork W2027864396 @default.
- W4232821856 hasRelatedWork W2042023686 @default.
- W4232821856 hasRelatedWork W2314687714 @default.
- W4232821856 hasRelatedWork W2402508197 @default.
- W4232821856 hasRelatedWork W2417317414 @default.
- W4232821856 hasRelatedWork W2528346846 @default.
- W4232821856 hasRelatedWork W2553902830 @default.
- W4232821856 hasRelatedWork W4232821856 @default.
- W4232821856 hasRelatedWork W4239642707 @default.
- W4232821856 isParatext "false" @default.
- W4232821856 isRetracted "false" @default.
- W4232821856 workType "article" @default.