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- W4233077246 abstract "Abstract The transmission of the apscaviroid tentatively named apple chlorotic fruit spot viroid (ACFSVd) was investigated using a one-step reverse-transcription (RT) droplet digital PCR assay for absolute quantification of the viroid, followed by quantification of relative standard curves by RT-qPCR. Our results indicate that ACFSVd is effectively transmitted by grafting, budding and seeds. No transmission has yet been observed to the viroid-inoculated pome fruit species Pyrus sp. and Cydonia sp. ACFSVd was detected in viruliferous aphids ( Myzus persicae , Dysaphis plantaginea ) and in codling moths ( Cydia pomonella ). The viroid was also detected systemically in the infected hemiparasitic plant Viscum album subsp. album (mistletoe)." @default.
- W4233077246 created "2022-05-12" @default.
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- W4233077246 date "2020-07-08" @default.
- W4233077246 modified "2023-09-25" @default.
- W4233077246 title "Transmission studies of the newly described apple chlorotic fruit spot viroid using a combined RT-qPCR and droplet digital PCR approach" @default.
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- W4233077246 doi "https://doi.org/10.1007/s00705-020-04704-5" @default.
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