Matches in SemOpenAlex for { <https://semopenalex.org/work/W4233251175> ?p ?o ?g. }
Showing items 1 to 64 of
64
with 100 items per page.
- W4233251175 endingPage "73" @default.
- W4233251175 startingPage "61" @default.
- W4233251175 abstract "3-Dehydroshikimic acid (DHS), in addition to being a potent antioxidant, is the key hydroaromatic intermediate in the biocatalytic conversion of glucose into aromatic bioproducts and a variety of industrial chemicals. Microbial synthesis of DHS, like other intermediates in the common pathway of aromatic amino acid biosynthesis, has previously been examined only under shake flask conditions. In this account, synthesis of DHS using recombinant Escherichia coli constructs is examined in a fed-batch fermentor where glucose availability, oxygenation levels, and solution pH are controlled. DHS yields and titers are also determined by the activity of 3-deoxy-D-arabino-heptulosonic acid 7-phosphate (DAHP) synthase. This enzyme's expression levels, sensitivity to feedback inhibition, and the availability of its substrates, phosphoenolpyruvate (PEP) and D-erythrose 4-phosphate (E4P), dictate its in vivo activity. By combining fed-batch fermentor control with amplified expression of a feedback-insensitive isozyme of DAHP synthase and amplified expression of transketolase, DHS titers of 69 g/L were synthesized in 30% yield (mol/mol) from D-glucose. Significant concentrations of 3-dehydroquinic acid (6.8 g/L) and gallic acid (6.6 g/L) were synthesized in addition to DHS. The pronounced impact of transketolase overexpression, which increases E4P availability, on DHS titers and yields indicates that PEP availability is not a limiting factor under the fed-batch fermentor conditions employed. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 64: 61–73, 1999." @default.
- W4233251175 created "2022-05-12" @default.
- W4233251175 creator A5027067971 @default.
- W4233251175 creator A5041069829 @default.
- W4233251175 creator A5042427575 @default.
- W4233251175 creator A5048401608 @default.
- W4233251175 creator A5051363890 @default.
- W4233251175 date "1999-07-05" @default.
- W4233251175 modified "2023-09-26" @default.
- W4233251175 title "Fed‐batch fermentor synthesis of 3‐dehydroshikimic acid using recombinant Escherichia coli" @default.
- W4233251175 doi "https://doi.org/10.1002/(sici)1097-0290(19990705)64:1<61::aid-bit7>3.3.co;2-7" @default.
- W4233251175 hasPublicationYear "1999" @default.
- W4233251175 type Work @default.
- W4233251175 citedByCount "0" @default.
- W4233251175 crossrefType "journal-article" @default.
- W4233251175 hasAuthorship W4233251175A5027067971 @default.
- W4233251175 hasAuthorship W4233251175A5041069829 @default.
- W4233251175 hasAuthorship W4233251175A5042427575 @default.
- W4233251175 hasAuthorship W4233251175A5048401608 @default.
- W4233251175 hasAuthorship W4233251175A5051363890 @default.
- W4233251175 hasConcept C100544194 @default.
- W4233251175 hasConcept C103432212 @default.
- W4233251175 hasConcept C104317684 @default.
- W4233251175 hasConcept C143937172 @default.
- W4233251175 hasConcept C181199279 @default.
- W4233251175 hasConcept C185592680 @default.
- W4233251175 hasConcept C2777108408 @default.
- W4233251175 hasConcept C2910339780 @default.
- W4233251175 hasConcept C547475151 @default.
- W4233251175 hasConcept C553450214 @default.
- W4233251175 hasConcept C55493867 @default.
- W4233251175 hasConcept C86803240 @default.
- W4233251175 hasConceptScore W4233251175C100544194 @default.
- W4233251175 hasConceptScore W4233251175C103432212 @default.
- W4233251175 hasConceptScore W4233251175C104317684 @default.
- W4233251175 hasConceptScore W4233251175C143937172 @default.
- W4233251175 hasConceptScore W4233251175C181199279 @default.
- W4233251175 hasConceptScore W4233251175C185592680 @default.
- W4233251175 hasConceptScore W4233251175C2777108408 @default.
- W4233251175 hasConceptScore W4233251175C2910339780 @default.
- W4233251175 hasConceptScore W4233251175C547475151 @default.
- W4233251175 hasConceptScore W4233251175C553450214 @default.
- W4233251175 hasConceptScore W4233251175C55493867 @default.
- W4233251175 hasConceptScore W4233251175C86803240 @default.
- W4233251175 hasIssue "1" @default.
- W4233251175 hasLocation W42332511751 @default.
- W4233251175 hasOpenAccess W4233251175 @default.
- W4233251175 hasPrimaryLocation W42332511751 @default.
- W4233251175 hasRelatedWork W1465188717 @default.
- W4233251175 hasRelatedWork W1769442777 @default.
- W4233251175 hasRelatedWork W1990846267 @default.
- W4233251175 hasRelatedWork W1993092177 @default.
- W4233251175 hasRelatedWork W1995986374 @default.
- W4233251175 hasRelatedWork W2004033922 @default.
- W4233251175 hasRelatedWork W2050917233 @default.
- W4233251175 hasRelatedWork W2062828081 @default.
- W4233251175 hasRelatedWork W4206322088 @default.
- W4233251175 hasRelatedWork W4307054459 @default.
- W4233251175 hasVolume "64" @default.
- W4233251175 isParatext "false" @default.
- W4233251175 isRetracted "false" @default.
- W4233251175 workType "article" @default.