Matches in SemOpenAlex for { <https://semopenalex.org/work/W4233311311> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W4233311311 endingPage "835" @default.
- W4233311311 startingPage "830" @default.
- W4233311311 abstract "Human plasma proteins were separated by combining four types of two-dimensional electrophoresis (2-DE) techniques to obtain systematic information on proteins and their constituent polypeptides. A micro gel system was employed to facilitate the analysis. A plasma sample was first analyzed under nondenaturing conditions of electrophoresis (Type I 2-DE) to characterize the properties of proteins under physiological conditions. The sample was then analyzed, employing nondenaturing isoelectric focusing in the first dimension and sodium dodecyl sulfate (SDS) electrophoresis in the second dimension (Type II 2-DE), to study the dissociation of noncovalently bound protein subunits. In the third type of 2-DE (Type III 2-DE), proteins were separated by nondenaturing isoelectric focusing and treated with urea/mercaptoethanol/SDS and then subjected to second-dimension SDS electrophoresis, to study the dissociation of disulfide-bonded polypeptides. In the fourth type of 2-DE (Type IV 2-DE), the conditions of denaturing 2-DE were employed; the sample was treated with SDS-mercaptoethanol-urea-Nonidet P-40, separated by denaturing isoelectric focusing, and then subjected to SDS electrophoresis. The combined 2-DE technique will be useful to construct a comprehensive database of plasma proteins combining a “nondenaturing protein map” (a protein map) and a “denaturing protein map” (a polypeptide map)." @default.
- W4233311311 created "2022-05-12" @default.
- W4233311311 creator A5051948442 @default.
- W4233311311 creator A5073808541 @default.
- W4233311311 creator A5079667330 @default.
- W4233311311 date "1999-01-01" @default.
- W4233311311 modified "2023-10-14" @default.
- W4233311311 title "A nondenaturing protein map of human plasma proteins correlated with a denaturing polypeptide map combining techniques of micro two-dimensional gel electrophoresis" @default.
- W4233311311 doi "https://doi.org/10.1002/(sici)1522-2683(19990101)20:4/5<830::aid-elps830>3.3.co;2-l" @default.
- W4233311311 hasPublicationYear "1999" @default.
- W4233311311 type Work @default.
- W4233311311 citedByCount "0" @default.
- W4233311311 crossrefType "journal-article" @default.
- W4233311311 hasAuthorship W4233311311A5051948442 @default.
- W4233311311 hasAuthorship W4233311311A5073808541 @default.
- W4233311311 hasAuthorship W4233311311A5079667330 @default.
- W4233311311 hasConcept C101660505 @default.
- W4233311311 hasConcept C104317684 @default.
- W4233311311 hasConcept C116084860 @default.
- W4233311311 hasConcept C178596410 @default.
- W4233311311 hasConcept C181199279 @default.
- W4233311311 hasConcept C185592680 @default.
- W4233311311 hasConcept C2778913249 @default.
- W4233311311 hasConcept C2780365088 @default.
- W4233311311 hasConcept C35859425 @default.
- W4233311311 hasConcept C40250595 @default.
- W4233311311 hasConcept C43617362 @default.
- W4233311311 hasConcept C46111723 @default.
- W4233311311 hasConcept C47793336 @default.
- W4233311311 hasConcept C50476208 @default.
- W4233311311 hasConcept C55493867 @default.
- W4233311311 hasConcept C96294017 @default.
- W4233311311 hasConceptScore W4233311311C101660505 @default.
- W4233311311 hasConceptScore W4233311311C104317684 @default.
- W4233311311 hasConceptScore W4233311311C116084860 @default.
- W4233311311 hasConceptScore W4233311311C178596410 @default.
- W4233311311 hasConceptScore W4233311311C181199279 @default.
- W4233311311 hasConceptScore W4233311311C185592680 @default.
- W4233311311 hasConceptScore W4233311311C2778913249 @default.
- W4233311311 hasConceptScore W4233311311C2780365088 @default.
- W4233311311 hasConceptScore W4233311311C35859425 @default.
- W4233311311 hasConceptScore W4233311311C40250595 @default.
- W4233311311 hasConceptScore W4233311311C43617362 @default.
- W4233311311 hasConceptScore W4233311311C46111723 @default.
- W4233311311 hasConceptScore W4233311311C47793336 @default.
- W4233311311 hasConceptScore W4233311311C50476208 @default.
- W4233311311 hasConceptScore W4233311311C55493867 @default.
- W4233311311 hasConceptScore W4233311311C96294017 @default.
- W4233311311 hasIssue "4-5" @default.
- W4233311311 hasLocation W42333113111 @default.
- W4233311311 hasOpenAccess W4233311311 @default.
- W4233311311 hasPrimaryLocation W42333113111 @default.
- W4233311311 hasRelatedWork W2025974571 @default.
- W4233311311 hasRelatedWork W2104812728 @default.
- W4233311311 hasRelatedWork W2117141996 @default.
- W4233311311 hasRelatedWork W2401823812 @default.
- W4233311311 hasRelatedWork W3022002877 @default.
- W4233311311 hasRelatedWork W3025457057 @default.
- W4233311311 hasRelatedWork W329012231 @default.
- W4233311311 hasRelatedWork W4251472921 @default.
- W4233311311 hasRelatedWork W43134475 @default.
- W4233311311 hasRelatedWork W2188063711 @default.
- W4233311311 hasVolume "20" @default.
- W4233311311 isParatext "false" @default.
- W4233311311 isRetracted "false" @default.
- W4233311311 workType "article" @default.