Matches in SemOpenAlex for { <https://semopenalex.org/work/W4233447181> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W4233447181 endingPage "1789" @default.
- W4233447181 startingPage "1789" @default.
- W4233447181 abstract "Abstract von Willebrand factor (VWF) is a large adhesive glycoprotein synthesized in megakaryocytes and endothelial cells and stored in platelet a-granules and Weibel-Palade bodies respectively. It protects Factor VIII (FVIII) from proteolysis and mediates the initial contact of platelets with the injured vessel wall thus playing an important role in hemostasis and thrombosis. VWF is crucial for the formation of occlusive thrombi at arterial shear rates. However, with only a few conflicting studies published, the role of VWF in venous thrombosis is still unclear. Therefore in order to understand the in vivo role of VWF and FVIII in experimental thrombosis under venous flow conditions, we decided to evaluate thrombosis in VWF−/−, FVIII−/− and transgenic mice lacking the GPIbα extracellular domain which was replaced by human Interleukin-4 receptor (IL4Rα/GPIbα-tg). In ferric chloride-injured veins, platelet adhesion to subendothelium is decreased and thrombus growth is impaired in the VWF−/− mice when compared to wild-type (WT). In the WT mice, thrombi grew to occlusive size with a mean time of 18 min and all injured venules occluded, whereas in VWF−/− mice none of the vessels occluded by 40 min after injury, when observation was terminated. Venules of mice deficient in FVIII treated similarly also did not occlude because of embolization. The infusion of recombinant human-FVIII (r-hu-FVIII) in FVIII−/− mice normalized the occlusion time to WT values. We also observed thrombus instability in the VWF−/− mice, which was due to lower FVIII levels in these mice since r-huFVIII restored thrombus stability i.e. prevented breaking of the thrombi with large platelet aggregates moving downstream. Despite normalization of blood clotting time and thrombus stability after r-FVIII infusion, the VWF−/− thrombi grew at a slower rate than WT and the venules did not occlude. In transgenic mice lacking the GPIbα extracellular domain, all injured venules occluded. Thus, VWF uses other adhesion receptors besides GPIbα in thrombus growth under venous shear conditions. Our studies document crucial independent roles for VWF and FVIII in experimental thrombosis under venous flow conditions in vivo." @default.
- W4233447181 created "2022-05-12" @default.
- W4233447181 creator A5012507110 @default.
- W4233447181 creator A5020480043 @default.
- W4233447181 creator A5032584210 @default.
- W4233447181 creator A5040756693 @default.
- W4233447181 creator A5049914754 @default.
- W4233447181 date "2006-11-16" @default.
- W4233447181 modified "2023-09-28" @default.
- W4233447181 title "von Willebrand Factor and Factor VIII Are Independently Required To Form Stable Occlusive Thrombi in Injured Veins." @default.
- W4233447181 doi "https://doi.org/10.1182/blood.v108.11.1789.1789" @default.
- W4233447181 hasPublicationYear "2006" @default.
- W4233447181 type Work @default.
- W4233447181 citedByCount "0" @default.
- W4233447181 crossrefType "journal-article" @default.
- W4233447181 hasAuthorship W4233447181A5012507110 @default.
- W4233447181 hasAuthorship W4233447181A5020480043 @default.
- W4233447181 hasAuthorship W4233447181A5032584210 @default.
- W4233447181 hasAuthorship W4233447181A5040756693 @default.
- W4233447181 hasAuthorship W4233447181A5049914754 @default.
- W4233447181 hasConcept C126322002 @default.
- W4233447181 hasConcept C134018914 @default.
- W4233447181 hasConcept C142724271 @default.
- W4233447181 hasConcept C185592680 @default.
- W4233447181 hasConcept C203014093 @default.
- W4233447181 hasConcept C2776992346 @default.
- W4233447181 hasConcept C2778589496 @default.
- W4233447181 hasConcept C2778780528 @default.
- W4233447181 hasConcept C2779216453 @default.
- W4233447181 hasConcept C2779394231 @default.
- W4233447181 hasConcept C2780009322 @default.
- W4233447181 hasConcept C2780868729 @default.
- W4233447181 hasConcept C2780921031 @default.
- W4233447181 hasConcept C2781362458 @default.
- W4233447181 hasConcept C2910597130 @default.
- W4233447181 hasConcept C2993802102 @default.
- W4233447181 hasConcept C71924100 @default.
- W4233447181 hasConcept C89560881 @default.
- W4233447181 hasConceptScore W4233447181C126322002 @default.
- W4233447181 hasConceptScore W4233447181C134018914 @default.
- W4233447181 hasConceptScore W4233447181C142724271 @default.
- W4233447181 hasConceptScore W4233447181C185592680 @default.
- W4233447181 hasConceptScore W4233447181C203014093 @default.
- W4233447181 hasConceptScore W4233447181C2776992346 @default.
- W4233447181 hasConceptScore W4233447181C2778589496 @default.
- W4233447181 hasConceptScore W4233447181C2778780528 @default.
- W4233447181 hasConceptScore W4233447181C2779216453 @default.
- W4233447181 hasConceptScore W4233447181C2779394231 @default.
- W4233447181 hasConceptScore W4233447181C2780009322 @default.
- W4233447181 hasConceptScore W4233447181C2780868729 @default.
- W4233447181 hasConceptScore W4233447181C2780921031 @default.
- W4233447181 hasConceptScore W4233447181C2781362458 @default.
- W4233447181 hasConceptScore W4233447181C2910597130 @default.
- W4233447181 hasConceptScore W4233447181C2993802102 @default.
- W4233447181 hasConceptScore W4233447181C71924100 @default.
- W4233447181 hasConceptScore W4233447181C89560881 @default.
- W4233447181 hasIssue "11" @default.
- W4233447181 hasLocation W42334471811 @default.
- W4233447181 hasOpenAccess W4233447181 @default.
- W4233447181 hasPrimaryLocation W42334471811 @default.
- W4233447181 hasRelatedWork W184289008 @default.
- W4233447181 hasRelatedWork W1991815296 @default.
- W4233447181 hasRelatedWork W2017376679 @default.
- W4233447181 hasRelatedWork W2156473494 @default.
- W4233447181 hasRelatedWork W2337950978 @default.
- W4233447181 hasRelatedWork W2474982893 @default.
- W4233447181 hasRelatedWork W3095326501 @default.
- W4233447181 hasRelatedWork W3176179351 @default.
- W4233447181 hasRelatedWork W4233447181 @default.
- W4233447181 hasRelatedWork W4234373395 @default.
- W4233447181 hasVolume "108" @default.
- W4233447181 isParatext "false" @default.
- W4233447181 isRetracted "false" @default.
- W4233447181 workType "article" @default.