Matches in SemOpenAlex for { <https://semopenalex.org/work/W4233470841> ?p ?o ?g. }
Showing items 1 to 59 of
59
with 100 items per page.
- W4233470841 endingPage "46" @default.
- W4233470841 startingPage "37" @default.
- W4233470841 abstract "An autonomously replicating shuttle vector was used to investigate enhancement of plasmid-chromosome recombination in mammalian host cells by gamma irradiation and UV light. Sequences homologous to the shuttle vector were stably inserted into the genome of African green monkey kidney cells to act as the target substrate for these recombination events. The shuttle vector molecules were irradiated at various doses before transfection into the mammalian host cells that contained the stable insertions. The homologous transfer of the bacterial ampicillin resistance gene from the inserted sequences to replace a mutant ampicillin sensitivity gene on the shuttle vector was identified by the recovery of ampicillin-resistant plasmids after Hirt extraction and transformation into Escherichia coli host cells. Gamma irradiation increased homologous shuttle vector-chromosome recombination, whereas UV light did not increase the frequency of recombinant plasmids detected. Introducing specific double-strand breaks in the plasmid or prolonging the time of plasmid residence in the mammalian host cells also enhanced plasmid-chromosome recombination. In contrast, plasmid mutagenesis was increased by UV irradiation of the plasmid but did not change with time. The ampicillin-resistant recombinant plasmid molecules analyzed appeared to rise mostly from nonconservative exchanges that involved both homologous and possibly nonhomologous interactions with the host chromosome. The observation that similar recombinant structures were obtained from all the plasmid treatments and host cells used suggests a common mechanism for plasmid-chromosome recombination in these mammalian cells." @default.
- W4233470841 created "2022-05-12" @default.
- W4233470841 creator A5069602023 @default.
- W4233470841 creator A5088449591 @default.
- W4233470841 date "1990-01-01" @default.
- W4233470841 modified "2023-10-12" @default.
- W4233470841 title "Recombination between irradiated shuttle vector DNA and chromosomal DNA in African green monkey kidney cells." @default.
- W4233470841 doi "https://doi.org/10.1128/mcb.10.1.37" @default.
- W4233470841 hasPublicationYear "1990" @default.
- W4233470841 type Work @default.
- W4233470841 citedByCount "14" @default.
- W4233470841 crossrefType "journal-article" @default.
- W4233470841 hasAuthorship W4233470841A5069602023 @default.
- W4233470841 hasAuthorship W4233470841A5088449591 @default.
- W4233470841 hasBestOaLocation W42334708411 @default.
- W4233470841 hasConcept C102744134 @default.
- W4233470841 hasConcept C104317684 @default.
- W4233470841 hasConcept C145640855 @default.
- W4233470841 hasConcept C153911025 @default.
- W4233470841 hasConcept C22744801 @default.
- W4233470841 hasConcept C40767141 @default.
- W4233470841 hasConcept C54355233 @default.
- W4233470841 hasConcept C552990157 @default.
- W4233470841 hasConcept C80343103 @default.
- W4233470841 hasConcept C86803240 @default.
- W4233470841 hasConcept C92087593 @default.
- W4233470841 hasConceptScore W4233470841C102744134 @default.
- W4233470841 hasConceptScore W4233470841C104317684 @default.
- W4233470841 hasConceptScore W4233470841C145640855 @default.
- W4233470841 hasConceptScore W4233470841C153911025 @default.
- W4233470841 hasConceptScore W4233470841C22744801 @default.
- W4233470841 hasConceptScore W4233470841C40767141 @default.
- W4233470841 hasConceptScore W4233470841C54355233 @default.
- W4233470841 hasConceptScore W4233470841C552990157 @default.
- W4233470841 hasConceptScore W4233470841C80343103 @default.
- W4233470841 hasConceptScore W4233470841C86803240 @default.
- W4233470841 hasConceptScore W4233470841C92087593 @default.
- W4233470841 hasIssue "1" @default.
- W4233470841 hasLocation W42334708411 @default.
- W4233470841 hasLocation W42334708412 @default.
- W4233470841 hasLocation W42334708413 @default.
- W4233470841 hasOpenAccess W4233470841 @default.
- W4233470841 hasPrimaryLocation W42334708411 @default.
- W4233470841 hasRelatedWork W1795846146 @default.
- W4233470841 hasRelatedWork W2082694669 @default.
- W4233470841 hasRelatedWork W2350023942 @default.
- W4233470841 hasRelatedWork W2356585887 @default.
- W4233470841 hasRelatedWork W2369286053 @default.
- W4233470841 hasRelatedWork W2373840057 @default.
- W4233470841 hasRelatedWork W2374703656 @default.
- W4233470841 hasRelatedWork W2978753736 @default.
- W4233470841 hasRelatedWork W4251805674 @default.
- W4233470841 hasRelatedWork W103609111 @default.
- W4233470841 hasVolume "10" @default.
- W4233470841 isParatext "false" @default.
- W4233470841 isRetracted "false" @default.
- W4233470841 workType "article" @default.