Matches in SemOpenAlex for { <https://semopenalex.org/work/W4233587512> ?p ?o ?g. }
Showing items 1 to 54 of
54
with 100 items per page.
- W4233587512 abstract "Increases in ligand binding to integrins (“activation”) play critical roles in platelet and leukocyte function. Integrin activation requires talin and kindlin binding to integrin β cytoplasmic tails. Much research has focused on the conserved GFFKR motif in integrin αIIb tails for its importance in keeping integrins inactive and integrin β cytoplasmic tails and their interacting partners. However, the roles of αIIb tail distal of GFFKR motif are unexplored. Here, we examine the role of αIIb tail distal of GFFKR in talin-mediated inside-out integrin activation, αIIbβ3 outside-in signaling and αIIbβ3-talin interactions. Deletion of amino acid residues after the GFFKR motif in αIIb tail abolished talin-induced inside-out αIIbβ3 activation without affecting αIIbβ3-talin interaction or outside-in αIIbβ3 signaling in modeled cell systems, measured by cell spreading and Src phosphorylation. Addition of non-homologous or non-specific amino acids to the GFFKR motif restored the capacity of talin to activate αIIbβ3 in modeled cells. Moreover, thrombin-stimulated αIIbβ3 activation in megakaryocytic leukemia cells (CMK cells) are similarly abolished by truncation after GFFKR and restored by adding non-specific sequences. Furthermore, Molecular modeling indicates that β3-bound talin sterically clashes with the αIIb tail in the αIIbβ3 complexes, potentially disfavoring the α-β interactions that keep αIIbβ3 inactive. Thus, the αIIb tail sequences distal of GFFKR participate in talin-mediated inside-out αIIbβ3 activation through its steric clashes with β3-bound talin." @default.
- W4233587512 created "2022-05-12" @default.
- W4233587512 creator A5015556167 @default.
- W4233587512 creator A5050311492 @default.
- W4233587512 creator A5059605240 @default.
- W4233587512 creator A5066584624 @default.
- W4233587512 date "2014-05-01" @default.
- W4233587512 modified "2023-09-27" @default.
- W4233587512 title "Abstract 24: Integrin αIIb Tail Participates in Inside-Out αIIbß3 Activation by Providing a Steric Lever Arm for Talin" @default.
- W4233587512 doi "https://doi.org/10.1161/atvb.34.suppl_1.24" @default.
- W4233587512 hasPublicationYear "2014" @default.
- W4233587512 type Work @default.
- W4233587512 citedByCount "0" @default.
- W4233587512 crossrefType "journal-article" @default.
- W4233587512 hasAuthorship W4233587512A5015556167 @default.
- W4233587512 hasAuthorship W4233587512A5050311492 @default.
- W4233587512 hasAuthorship W4233587512A5059605240 @default.
- W4233587512 hasAuthorship W4233587512A5066584624 @default.
- W4233587512 hasConcept C11960822 @default.
- W4233587512 hasConcept C1491633281 @default.
- W4233587512 hasConcept C185592680 @default.
- W4233587512 hasConcept C190062978 @default.
- W4233587512 hasConcept C195687474 @default.
- W4233587512 hasConcept C2910542480 @default.
- W4233587512 hasConcept C55493867 @default.
- W4233587512 hasConcept C86803240 @default.
- W4233587512 hasConcept C95444343 @default.
- W4233587512 hasConceptScore W4233587512C11960822 @default.
- W4233587512 hasConceptScore W4233587512C1491633281 @default.
- W4233587512 hasConceptScore W4233587512C185592680 @default.
- W4233587512 hasConceptScore W4233587512C190062978 @default.
- W4233587512 hasConceptScore W4233587512C195687474 @default.
- W4233587512 hasConceptScore W4233587512C2910542480 @default.
- W4233587512 hasConceptScore W4233587512C55493867 @default.
- W4233587512 hasConceptScore W4233587512C86803240 @default.
- W4233587512 hasConceptScore W4233587512C95444343 @default.
- W4233587512 hasIssue "suppl_1" @default.
- W4233587512 hasLocation W42335875121 @default.
- W4233587512 hasOpenAccess W4233587512 @default.
- W4233587512 hasPrimaryLocation W42335875121 @default.
- W4233587512 hasRelatedWork W1333366 @default.
- W4233587512 hasRelatedWork W13948506 @default.
- W4233587512 hasRelatedWork W14361505 @default.
- W4233587512 hasRelatedWork W2400917 @default.
- W4233587512 hasRelatedWork W25672997 @default.
- W4233587512 hasRelatedWork W47387614 @default.
- W4233587512 hasRelatedWork W4760336 @default.
- W4233587512 hasRelatedWork W5422456 @default.
- W4233587512 hasRelatedWork W8235269 @default.
- W4233587512 hasRelatedWork W9781973 @default.
- W4233587512 hasVolume "34" @default.
- W4233587512 isParatext "false" @default.
- W4233587512 isRetracted "false" @default.
- W4233587512 workType "article" @default.