Matches in SemOpenAlex for { <https://semopenalex.org/work/W4233660117> ?p ?o ?g. }
Showing items 1 to 59 of
59
with 100 items per page.
- W4233660117 abstract "Abstract Background: Kawasaki disease (KD) is the most common pediatric systemic vasculitides of unknown etiology. Recent clinical studies led to reappraisal of the usefulness of initial combination therapy of intravenous immunoglobulin (IVIG) plus a corticosteroid for patients with severe KD. However, the molecular mechanisms underlying the clinical benefits of early introduction of a corticosteroid to IVIG for severe KD patients remain unclear. We used cultured human coronary artery endothelial cells (HCAECs), which mimic the main lesion sites of KD, in an attempt to elucidate the mechanisms underlying the clinical benefits accruing from adding a corticosteroid to standard IVIG therapy for patients with KD. Methods: HCAECs were stimulated with TNF-α, IL-1α or IL-1β in the presence and absence of IVIG and/or dexamethasone (DEX). The mRNA and protein concentrations for high-mobility group box-1 (HMGB1), IL-1α, IL-6 and granulocyte-colony stimulating factor (G-CSF) in the culture supernatants were measured by quantitative PCR (qPCR) and ELISA, respectively. Apoptosis was evaluated by the caspase 3/7 activities. Results: DEX, but not IVIG, significantly inhibited apoptosis caused by inflammatory stimuli, resulting in effective reduction of HMGB1 and IL-1α protein release by HCAECs. As previously reported, DEX or IVIG alone significantly suppressed TNF-α-induced production of IL-6 and G-CSF and mRNA expression, but induction of those cytokines by IL-1s (IL-1α and IL-1β) was resistant to IVIG. Conclusions: A corticosteroid can effectively inhibit the release of HMGB1 and IL-1α, which may be involved in IVIG resistance in KD. Since IVIG does not have such beneficial anti-cytotoxic effects, adding a corticosteroid to standard IVIG therapy may help prevent the progression of IVIG resistance in KD." @default.
- W4233660117 created "2022-05-12" @default.
- W4233660117 creator A5005332967 @default.
- W4233660117 creator A5032830362 @default.
- W4233660117 creator A5072564461 @default.
- W4233660117 creator A5079099744 @default.
- W4233660117 date "2020-03-10" @default.
- W4233660117 modified "2023-09-25" @default.
- W4233660117 title "Functional benefits of corticosteroid and IVIG combination therapy in a coronary artery endothelial cell model of Kawasaki disease" @default.
- W4233660117 doi "https://doi.org/10.21203/rs.3.rs-16532/v1" @default.
- W4233660117 hasPublicationYear "2020" @default.
- W4233660117 type Work @default.
- W4233660117 citedByCount "0" @default.
- W4233660117 crossrefType "posted-content" @default.
- W4233660117 hasAuthorship W4233660117A5005332967 @default.
- W4233660117 hasAuthorship W4233660117A5032830362 @default.
- W4233660117 hasAuthorship W4233660117A5072564461 @default.
- W4233660117 hasAuthorship W4233660117A5079099744 @default.
- W4233660117 hasBestOaLocation W42336601171 @default.
- W4233660117 hasConcept C126322002 @default.
- W4233660117 hasConcept C159654299 @default.
- W4233660117 hasConcept C203014093 @default.
- W4233660117 hasConcept C2776804153 @default.
- W4233660117 hasConcept C2776820930 @default.
- W4233660117 hasConcept C2776914184 @default.
- W4233660117 hasConcept C2778213512 @default.
- W4233660117 hasConcept C2780401358 @default.
- W4233660117 hasConcept C2780545709 @default.
- W4233660117 hasConcept C2781423770 @default.
- W4233660117 hasConcept C71924100 @default.
- W4233660117 hasConceptScore W4233660117C126322002 @default.
- W4233660117 hasConceptScore W4233660117C159654299 @default.
- W4233660117 hasConceptScore W4233660117C203014093 @default.
- W4233660117 hasConceptScore W4233660117C2776804153 @default.
- W4233660117 hasConceptScore W4233660117C2776820930 @default.
- W4233660117 hasConceptScore W4233660117C2776914184 @default.
- W4233660117 hasConceptScore W4233660117C2778213512 @default.
- W4233660117 hasConceptScore W4233660117C2780401358 @default.
- W4233660117 hasConceptScore W4233660117C2780545709 @default.
- W4233660117 hasConceptScore W4233660117C2781423770 @default.
- W4233660117 hasConceptScore W4233660117C71924100 @default.
- W4233660117 hasLocation W42336601171 @default.
- W4233660117 hasLocation W42336601172 @default.
- W4233660117 hasLocation W42336601173 @default.
- W4233660117 hasOpenAccess W4233660117 @default.
- W4233660117 hasPrimaryLocation W42336601171 @default.
- W4233660117 hasRelatedWork W1115928 @default.
- W4233660117 hasRelatedWork W12178806 @default.
- W4233660117 hasRelatedWork W12652107 @default.
- W4233660117 hasRelatedWork W14013891 @default.
- W4233660117 hasRelatedWork W16353228 @default.
- W4233660117 hasRelatedWork W332685 @default.
- W4233660117 hasRelatedWork W429484 @default.
- W4233660117 hasRelatedWork W6544046 @default.
- W4233660117 hasRelatedWork W7825235 @default.
- W4233660117 hasRelatedWork W9670700 @default.
- W4233660117 isParatext "false" @default.
- W4233660117 isRetracted "false" @default.
- W4233660117 workType "article" @default.