FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4234093616> ?p ?o ?g. }

• W4234093616 endingPage "66" @default.
• W4234093616 startingPage "55" @default.
• W4234093616 abstract "ABSTRACT Herpes simplex virus DNA polymerase consists of a catalytic subunit, Pol, and a processivity subunit, UL42, that, unlike other established processivity factors, binds DNA directly. We used gel retardation and filter-binding assays to investigate how UL42 affects the polymerase-DNA interaction. The Pol/UL42 heterodimer bound more tightly to DNA in a primer-template configuration than to single-stranded DNA (ssDNA), while Pol alone bound more tightly to ssDNA than to DNA in a primer-template configuration. The affinity of Pol/UL42 for ssDNA was reduced severalfold relative to that of Pol, while the affinity of Pol/UL42 for primer-template DNA was increased ∼15-fold relative to that of Pol. The affinity of Pol/UL42 for circular double-stranded DNA (dsDNA) was reduced drastically relative to that of UL42, but the affinity of Pol/UL42 for short primer-templates was increased modestly relative to that of UL42. Pol/UL42 associated with primer-template DNA ∼2-fold faster than did Pol and dissociated ∼10-fold more slowly, resulting in a half-life of 2 h and a subnanomolar K d . Despite such stable binding, rapid-quench analysis revealed that the rates of elongation of Pol/UL42 and Pol were essentially the same, ∼30 nucleotides/s. Taken together, these studies indicate that (i) Pol/UL42 is more likely than its subunits to associate with DNA in a primer-template configuration rather than nonspecifically to either ssDNA or dsDNA, and (ii) UL42 reduces the rate of dissociation from primer-template DNA but not the rate of elongation. Two models of polymerase-DNA interactions during replication that may explain these findings are presented." @default.
• W4234093616 created "2022-05-12" @default.
• W4234093616 creator A5030810079 @default.
• W4234093616 creator A5061147470 @default.
• W4234093616 creator A5067518378 @default.
• W4234093616 date "1999-01-01" @default.
• W4234093616 modified "2023-10-18" @default.
• W4234093616 title "Herpes Simplex Virus Processivity Factor UL42 Imparts Increased DNA-Binding Specificity to the Viral DNA Polymerase and Decreased Dissociation from Primer-Template without Reducing the Elongation Rate" @default.
• W4234093616 cites W1211711083 @default.
• W4234093616 cites W1495349824 @default.
• W4234093616 cites W1496831544 @default.
• W4234093616 cites W1501357974 @default.
• W4234093616 cites W1502391241 @default.
• W4234093616 cites W1509692938 @default.
• W4234093616 cites W1512885164 @default.
• W4234093616 cites W1526424212 @default.
• W4234093616 cites W1526506014 @default.
• W4234093616 cites W1542131890 @default.
• W4234093616 cites W1545377574 @default.
• W4234093616 cites W1546917481 @default.
• W4234093616 cites W1547854436 @default.
• W4234093616 cites W1551254087 @default.
• W4234093616 cites W1563147703 @default.
• W4234093616 cites W1569045429 @default.
• W4234093616 cites W1572163981 @default.
• W4234093616 cites W1587132435 @default.
• W4234093616 cites W1589132235 @default.
• W4234093616 cites W1597008099 @default.
• W4234093616 cites W1634217411 @default.
• W4234093616 cites W1661691401 @default.
• W4234093616 cites W1670236664 @default.
• W4234093616 cites W1911004472 @default.
• W4234093616 cites W1978167050 @default.
• W4234093616 cites W1979989270 @default.
• W4234093616 cites W1982845316 @default.
• W4234093616 cites W1988840026 @default.
• W4234093616 cites W1999925341 @default.
• W4234093616 cites W2009301554 @default.
• W4234093616 cites W2020390932 @default.
• W4234093616 cites W2023461942 @default.
• W4234093616 cites W2026321529 @default.
• W4234093616 cites W2028663881 @default.
• W4234093616 cites W2029309152 @default.
• W4234093616 cites W2041249795 @default.
• W4234093616 cites W2056650797 @default.
• W4234093616 cites W2056730104 @default.
• W4234093616 cites W2060769308 @default.
• W4234093616 cites W2065014822 @default.
• W4234093616 cites W2071887319 @default.
• W4234093616 cites W2072768518 @default.
• W4234093616 cites W2079718256 @default.
• W4234093616 cites W2085873646 @default.
• W4234093616 cites W2088644418 @default.
• W4234093616 cites W2091172849 @default.
• W4234093616 cites W2095333277 @default.
• W4234093616 cites W2110646721 @default.
• W4234093616 cites W2111745935 @default.
• W4234093616 cites W2116537286 @default.
• W4234093616 cites W2122748184 @default.
• W4234093616 cites W2128290262 @default.
• W4234093616 cites W2137707694 @default.
• W4234093616 cites W2210677056 @default.
• W4234093616 doi "https://doi.org/10.1128/jvi.73.1.55-66.1999" @default.
• W4234093616 hasPublicationYear "1999" @default.
• W4234093616 type Work @default.
• W4234093616 citedByCount "46" @default.
• W4234093616 countsByYear W42340936162015 @default.
• W4234093616 countsByYear W42340936162017 @default.
• W4234093616 countsByYear W42340936162018 @default.
• W4234093616 countsByYear W42340936162019 @default.
• W4234093616 countsByYear W42340936162020 @default.
• W4234093616 countsByYear W42340936162021 @default.
• W4234093616 countsByYear W42340936162022 @default.
• W4234093616 crossrefType "journal-article" @default.
• W4234093616 hasAuthorship W4234093616A5030810079 @default.
• W4234093616 hasAuthorship W4234093616A5061147470 @default.
• W4234093616 hasAuthorship W4234093616A5067518378 @default.
• W4234093616 hasBestOaLocation W42340936161 @default.
• W4234093616 hasConcept C104317684 @default.
• W4234093616 hasConcept C12554922 @default.
• W4234093616 hasConcept C131140672 @default.
• W4234093616 hasConcept C153911025 @default.
• W4234093616 hasConcept C156719811 @default.
• W4234093616 hasConcept C178790620 @default.
• W4234093616 hasConcept C185592680 @default.
• W4234093616 hasConcept C2756471 @default.
• W4234093616 hasConcept C2777563447 @default.
• W4234093616 hasConcept C31254050 @default.
• W4234093616 hasConcept C552990157 @default.
• W4234093616 hasConcept C55493867 @default.
• W4234093616 hasConcept C67705224 @default.
• W4234093616 hasConcept C76535049 @default.
• W4234093616 hasConcept C82381507 @default.
• W4234093616 hasConcept C86803240 @default.
• W4234093616 hasConcept C88354546 @default.
• W4234093616 hasConceptScore W4234093616C104317684 @default.
• W4234093616 hasConceptScore W4234093616C12554922 @default.
• W4234093616 hasConceptScore W4234093616C131140672 @default.

• next