FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4234338924> ?p ?o ?g. }

• W4234338924 endingPage "2513" @default.
• W4234338924 startingPage "2501" @default.
• W4234338924 abstract "The peripherin gene, which encodes a neuronal-specific intermediate filament protein, is transcriptionally induced with a late time course when nerve growth factor (NGF) stimulates PC12 cells to differentiate into neurons. We have studied its transcriptional regulation in order to better understand the neuronal-specific end steps of the signal transduction pathway of NGF. By 5' deletion mapping of the peripherin promoter, we have localized two positive regulatory elements necessary for full induction by NGF: a distal positive element and a proximal constitutive element within 111 bp of the transcriptional start site. In addition, there is a negative regulatory element (NRE; -179 to -111), the deletion of which results in elevated basal expression of the gene. Methylation interference footprinting of the NRE defined a unique sequence, GGCAGGGCGCC, as the binding site for proteins present in nuclear extracts from both undifferentiated and differentiated PC12 cells. However, DNA mobility shift assays using an oligonucleotide probe containing the footprinted sequence demonstrate a prominent retarded complex in extracts from undifferentiated PC12 cells which migrates with slower mobility than do the complexes produced by using differentiated PC12 cell extract. Transfection experiments using peripherin-chloramphenicol acetyltransferase constructs in which the footprinted sequence has been mutated confirm that the NRE has a functional, though not exclusive, role in repressing peripherin expression in undifferentiated and nonneuronal cells. We propose a two-step model of activation of peripherin by NGF in which dissociation of a repressor from the protein complex at the NRE, coupled with a positive signal from the distal positive element, results in depression of the gene." @default.
• W4234338924 created "2022-05-12" @default.
• W4234338924 creator A5005293193 @default.
• W4234338924 creator A5038344661 @default.
• W4234338924 creator A5069425192 @default.
• W4234338924 creator A5070242162 @default.
• W4234338924 creator A5088204152 @default.
• W4234338924 date "1992-06-01" @default.
• W4234338924 modified "2023-09-27" @default.
• W4234338924 title "Nerve growth factor-induced derepression of peripherin gene expression is associated with alterations in proteins binding to a negative regulatory element" @default.
• W4234338924 cites W122840240 @default.
• W4234338924 cites W1430328139 @default.
• W4234338924 cites W1500220781 @default.
• W4234338924 cites W1531884888 @default.
• W4234338924 cites W1550056485 @default.
• W4234338924 cites W1582530932 @default.
• W4234338924 cites W1592357554 @default.
• W4234338924 cites W1592463385 @default.
• W4234338924 cites W1593454126 @default.
• W4234338924 cites W1634432451 @default.
• W4234338924 cites W1853725381 @default.
• W4234338924 cites W1941968244 @default.
• W4234338924 cites W1970587959 @default.
• W4234338924 cites W1971436037 @default.
• W4234338924 cites W1973444714 @default.
• W4234338924 cites W1981881657 @default.
• W4234338924 cites W1982387821 @default.
• W4234338924 cites W1982910659 @default.
• W4234338924 cites W1984767179 @default.
• W4234338924 cites W1985300178 @default.
• W4234338924 cites W1985822867 @default.
• W4234338924 cites W1989326240 @default.
• W4234338924 cites W1989555252 @default.
• W4234338924 cites W1990493528 @default.
• W4234338924 cites W1993243592 @default.
• W4234338924 cites W1994460947 @default.
• W4234338924 cites W1994852189 @default.
• W4234338924 cites W1995646607 @default.
• W4234338924 cites W2004179558 @default.
• W4234338924 cites W2005929965 @default.
• W4234338924 cites W2006164030 @default.
• W4234338924 cites W2010488597 @default.
• W4234338924 cites W2012760766 @default.
• W4234338924 cites W2015379420 @default.
• W4234338924 cites W2021235665 @default.
• W4234338924 cites W2021253208 @default.
• W4234338924 cites W2036017389 @default.
• W4234338924 cites W2036931150 @default.
• W4234338924 cites W2042458199 @default.
• W4234338924 cites W2050606490 @default.
• W4234338924 cites W2053572117 @default.
• W4234338924 cites W2054264055 @default.
• W4234338924 cites W2054468433 @default.
• W4234338924 cites W2055540751 @default.
• W4234338924 cites W2073611916 @default.
• W4234338924 cites W2074563618 @default.
• W4234338924 cites W2077492124 @default.
• W4234338924 cites W2078119356 @default.
• W4234338924 cites W2078146089 @default.
• W4234338924 cites W2091309613 @default.
• W4234338924 cites W2092451011 @default.
• W4234338924 cites W2115736681 @default.
• W4234338924 cites W2143265447 @default.
• W4234338924 cites W2148071983 @default.
• W4234338924 cites W2163287185 @default.
• W4234338924 cites W2166801871 @default.
• W4234338924 cites W252338653 @default.
• W4234338924 cites W3105898142 @default.
• W4234338924 cites W4237021103 @default.
• W4234338924 cites W4248096216 @default.
• W4234338924 cites W948550917 @default.
• W4234338924 doi "https://doi.org/10.1128/mcb.12.6.2501-2513.1992" @default.
• W4234338924 hasPublicationYear "1992" @default.
• W4234338924 type Work @default.
• W4234338924 citedByCount "1" @default.
• W4234338924 crossrefType "journal-article" @default.
• W4234338924 hasAuthorship W4234338924A5005293193 @default.
• W4234338924 hasAuthorship W4234338924A5038344661 @default.
• W4234338924 hasAuthorship W4234338924A5069425192 @default.
• W4234338924 hasAuthorship W4234338924A5070242162 @default.
• W4234338924 hasAuthorship W4234338924A5088204152 @default.
• W4234338924 hasConcept C104317684 @default.
• W4234338924 hasConcept C153911025 @default.
• W4234338924 hasConcept C158448853 @default.
• W4234338924 hasConcept C21592294 @default.
• W4234338924 hasConcept C2778061764 @default.
• W4234338924 hasConcept C2778994924 @default.
• W4234338924 hasConcept C28521208 @default.
• W4234338924 hasConcept C54355233 @default.
• W4234338924 hasConcept C86339819 @default.
• W4234338924 hasConcept C86803240 @default.
• W4234338924 hasConcept C95444343 @default.
• W4234338924 hasConceptScore W4234338924C104317684 @default.
• W4234338924 hasConceptScore W4234338924C153911025 @default.
• W4234338924 hasConceptScore W4234338924C158448853 @default.
• W4234338924 hasConceptScore W4234338924C21592294 @default.
• W4234338924 hasConceptScore W4234338924C2778061764 @default.
• W4234338924 hasConceptScore W4234338924C2778994924 @default.

• next