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- W4234378627 abstract "A GDH gene from Halobacterium salinarum has been cloned and sequenced and the publication assigns the sequence to the NADP+-glutamate dehydrogenase of this organism. We have expressed this gene in Escherichia coli and find that it encodes an NAD+-dependent glutamate dehydrogenase without activity towards NADP+. Further, peptide sequence from the two corresponding proteins supports the view that the deposited sequence is indeed that of the NAD+-dependent glutamate dehydrogenase. Sequence from the NAD+-dependent protein matches the published gene sequence, whereas sequence from the NADP+ glutamate dehydrogenase does not." @default.
- W4234378627 created "2022-05-12" @default.
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- W4234378627 date "2002-05-21" @default.
- W4234378627 modified "2023-09-26" @default.
- W4234378627 title "Glutamate dehydrogenase of Halobacterium salinarum: evidence that the gene sequence currently assigned to the NADP+-dependent enzyme is in fact that of the NAD+-dependent glutamate dehydrogenase" @default.
- W4234378627 doi "https://doi.org/10.1016/s0378-1097(02)00644-4" @default.
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