Matches in SemOpenAlex for { <https://semopenalex.org/work/W4234544880> ?p ?o ?g. }
- W4234544880 endingPage "26136" @default.
- W4234544880 startingPage "26126" @default.
- W4234544880 abstract "Recombinant human erythropoietin (EPO) is the main therapeutic glycoprotein for the treatment of anemia in cancer and kidney patients. The in-vivo activity of EPO is carbohydrate-dependent with the number of sialic acid residues regulating its circulatory half-life. EPO carries three N-glycans and thus obtaining pure glycoforms provides a major challenge. We have developed a robust and reproducible chemoenzymatic approach to glycoforms of EPO with and without sialic acids. EPO was assembled by sequential native chemical ligation of two peptide and three glycopeptide segments. The glycopeptides were obtained by pseudoproline-assisted Lansbury aspartylation. Enzymatic introduction of the sialic acids was readily accomplished at the level of the glycopeptide segments but even more efficiently on the refolded glycoprotein. Biological recognition of the synthetic EPOs was shown by formation of 1:1 complexes with recombinant EPO receptor." @default.
- W4234544880 created "2022-05-12" @default.
- W4234544880 creator A5009144315 @default.
- W4234544880 creator A5010857300 @default.
- W4234544880 creator A5010962464 @default.
- W4234544880 creator A5018054349 @default.
- W4234544880 creator A5026435239 @default.
- W4234544880 creator A5037248577 @default.
- W4234544880 creator A5038503517 @default.
- W4234544880 creator A5053448579 @default.
- W4234544880 creator A5066091974 @default.
- W4234544880 creator A5069429386 @default.
- W4234544880 creator A5075875721 @default.
- W4234544880 creator A5080806454 @default.
- W4234544880 creator A5082288381 @default.
- W4234544880 date "2021-11-02" @default.
- W4234544880 modified "2023-09-27" @default.
- W4234544880 title "Chemical and Enzymatic Synthesis of Sialylated Glycoforms of Human Erythropoietin" @default.
- W4234544880 cites W1513042772 @default.
- W4234544880 cites W1863837456 @default.
- W4234544880 cites W1933659167 @default.
- W4234544880 cites W1971923734 @default.
- W4234544880 cites W1979379256 @default.
- W4234544880 cites W1981964101 @default.
- W4234544880 cites W1986436077 @default.
- W4234544880 cites W1993577968 @default.
- W4234544880 cites W1998152226 @default.
- W4234544880 cites W1998705721 @default.
- W4234544880 cites W2000015749 @default.
- W4234544880 cites W2002749607 @default.
- W4234544880 cites W2009899368 @default.
- W4234544880 cites W2012166707 @default.
- W4234544880 cites W2013563801 @default.
- W4234544880 cites W2016665838 @default.
- W4234544880 cites W2018894098 @default.
- W4234544880 cites W2021102059 @default.
- W4234544880 cites W2021482099 @default.
- W4234544880 cites W2029162393 @default.
- W4234544880 cites W2033255695 @default.
- W4234544880 cites W2037314853 @default.
- W4234544880 cites W2040764971 @default.
- W4234544880 cites W2041174313 @default.
- W4234544880 cites W2045301848 @default.
- W4234544880 cites W2058442860 @default.
- W4234544880 cites W2059124335 @default.
- W4234544880 cites W2065186793 @default.
- W4234544880 cites W2082276184 @default.
- W4234544880 cites W2086358459 @default.
- W4234544880 cites W2086627779 @default.
- W4234544880 cites W2088157674 @default.
- W4234544880 cites W2089453574 @default.
- W4234544880 cites W2090084711 @default.
- W4234544880 cites W2090263507 @default.
- W4234544880 cites W2097321089 @default.
- W4234544880 cites W2097598519 @default.
- W4234544880 cites W2099831601 @default.
- W4234544880 cites W2102893844 @default.
- W4234544880 cites W2106513137 @default.
- W4234544880 cites W2111235048 @default.
- W4234544880 cites W2115140577 @default.
- W4234544880 cites W2116179149 @default.
- W4234544880 cites W2126721880 @default.
- W4234544880 cites W2130820294 @default.
- W4234544880 cites W2133871435 @default.
- W4234544880 cites W2138995597 @default.
- W4234544880 cites W2144667100 @default.
- W4234544880 cites W2152991917 @default.
- W4234544880 cites W2156117820 @default.
- W4234544880 cites W2158758488 @default.
- W4234544880 cites W2179584440 @default.
- W4234544880 cites W2316760226 @default.
- W4234544880 cites W2321921836 @default.
- W4234544880 cites W2326385565 @default.
- W4234544880 cites W2334082320 @default.
- W4234544880 cites W2345064040 @default.
- W4234544880 cites W2419607388 @default.
- W4234544880 cites W2604521083 @default.
- W4234544880 cites W2609742448 @default.
- W4234544880 cites W2792072976 @default.
- W4234544880 cites W2898672591 @default.
- W4234544880 cites W2935851821 @default.
- W4234544880 cites W2951457835 @default.
- W4234544880 cites W2981074682 @default.
- W4234544880 cites W3021272140 @default.
- W4234544880 cites W3083501596 @default.
- W4234544880 cites W3110103462 @default.
- W4234544880 cites W3119251452 @default.
- W4234544880 cites W3144242447 @default.
- W4234544880 cites W4205791431 @default.
- W4234544880 cites W4213262935 @default.
- W4234544880 cites W4229628765 @default.
- W4234544880 cites W4230878475 @default.
- W4234544880 cites W4235156819 @default.
- W4234544880 cites W4239401066 @default.
- W4234544880 cites W4247047200 @default.
- W4234544880 cites W4247130211 @default.
- W4234544880 cites W4248329707 @default.
- W4234544880 cites W4248917954 @default.