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- W4234563784 abstract "Abstract The limitations of classical chromosome studies have been overcome by the introduction of fluorescence in situ hybridization (FISH), which offers a molecular dimension to cytogenetic analyses. It is a very useful technique for detection of targeted chromosomal abnormalities and applicable to many specimen types such as blood, bone marrow, tissue touch preparations, body fluids, and even paraffin‐embedded fixed tissue. Different FISH technologies have emerged, each with their own particular advantages and applications, for example, interphase FISH, whole‐chromosome paints, multicolor‐FISH, conventional comparative genome hybridization, and now array CGH. These techniques are all essentially based upon the annealing of single‐stranded probe DNA to the complementary DNA sequences, present in their natural position on the chromosomes (in situ hybridization). Rapid development of new types of probes such as cosmids, P1, PAC, BAC, YAC, oligos, microdissected chromosome regions, and whole‐chromosome libraries, dramatically increased the possible applications for FISH. Judicious choice from the molecular cytogenetic techniques now available has opened venues to novel applications, and consequently, even more information about cytogenetic abnormalities can now be obtained." @default.
- W4234563784 created "2022-05-12" @default.
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- W4234563784 date "2005-04-15" @default.
- W4234563784 modified "2023-10-18" @default.
- W4234563784 title "<scp>FISH</scp>" @default.
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- W4234563784 doi "https://doi.org/10.1002/047001153x.g102411" @default.
- W4234563784 hasPublicationYear "2005" @default.
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