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- W4234675560 abstract "Bone marrow mesenchymal stem cells are one of the important sources for cell replacement therapies. The outcome of the cell-based therapies is determined by a variety of factors including the source of the cells and their functional status. The functional status is usually measured by the expression of stem cell specific marker Oct-4 (stemness), differentiation potential (multipotency), and production of paracrine factors. It could be beneficial to keep the stem cells in a multipotent state for their optimal therapeutic outcome. The present study was to investigate the effect of basic fibroblast growth factor (bFGF) on the stemness of bone marrow stem cells and related mechanism. Rat multipotent adult progenitor cells (MAPCs) were used as the source of bone marrow stem cells, and induced to differentiate in vitro with (bFGF:5ng/ml and 20ng/ml) and without bFGF for up to 7 days. The expression of Oct4 and endothelial markers including Flk1, VWF and CD31, and smooth muscle cell markers including α-SMA, SM22 and CNN1 were determined with real-time (RT) PCR and western blot. We observed that both transcriptional (as reflected by RT-PCR) and protein expression (by western blot) of Oct4 was maintained at relatively stable levels in the cells treated with bFGF during the early phase of differentition. On the other hand, the expression of Flk1, VWF, CD31 and α-SMA, SM22, CNN1 were significantly decreased in the cells with the presence of bFGF. Treatment with bFGF significantly increased the phosphorylation of Erk1/2, not Akt nor STAT3, in the cells. Inhibition of Erk1/2 phosphorylation with PD98059 partially but significantly attenuated the effect of bFGF on Oct4 expression in the cells. These data suggest that bFGF could be able to maintain the bone marrow stem cells in their undifferentiated state in vitro partially through Erk1/2 signaling-mediated mechanism." @default.
- W4234675560 created "2022-05-12" @default.
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- W4234675560 date "2017-05-01" @default.
- W4234675560 modified "2023-09-24" @default.
- W4234675560 title "Abstract 621: Basic Fibroblast Growth Factor Helps to Maintain the Stemness of Rat Multipotent Adult Progenitor Cells Partially via Erk1/2 Signaling" @default.
- W4234675560 doi "https://doi.org/10.1161/atvb.37.suppl_1.621" @default.
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