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- W4234987900 abstract "Publisher SummaryThis chapter discusses the Maxam–Gilbert chemical procedure for DNA sequencing. Since its introduction in 1977, this procedure has become the most widely used sequencing procedure for DNA. In the chemical DNA sequencing method, end-labeled DNA is partially cleaved at each of the four nucleotide bases in four different reactions, the products ordered by size by gel electrophoresis and the sequence read-off an autoradiograph by noting the base-specific agent that cleaved at each successive nucleotide along the strand. No in vitro enzymatic copying is required and either single- or double-stranded DNA, labeled at either the 3′- or 5′-end, can be sequenced. In case of Maxam–Gilbert procedure, the crucial step is the specific chemically-induced cleavage at one or two of the four nucleotide bases. This is a three-stage process, involving modification of the base, removal of the modified base from its sugar, and finally strand scission at that sugar. Dimethylsulphate is an alkylating agent that methylates ring nitrogens in the DNA bases. As far as sequencing is concerned, the relevant methylation sites are the N7 position in guanine and the N3 in adenine." @default.
- W4234987900 created "2022-05-12" @default.
- W4234987900 date "1983-01-01" @default.
- W4234987900 modified "2023-10-16" @default.
- W4234987900 title "Chapter 5 DNA sequencing by the Maxam-Gilbert chemical procedure" @default.
- W4234987900 doi "https://doi.org/10.1016/s0075-7535(08)70411-x" @default.
- W4234987900 hasPublicationYear "1983" @default.
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