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- W4235981932 abstract "Many fluorescent proteins have been created to act as genetically encoded biosensors. With these sensors, changes in fluorescence report on biochemical states within living cells. Transition metal ions such as copper, nickel, and zinc are crucial in many physiological and pathophysiological pathways. Here, we engineered a spectral series of optimized transition metal ion-binding fluorescent proteins that respond to metals with large changes in fluorescence intensity. These proteins are useful as metal biosensors or tunable imaging probes. Each protein is uniquely modulated by four different metals (Cu2+, Ni2+, Co2+, and Zn2+). Crystallography revealed the geometry and location of metal binding to the engineered sites. When attached to an extracellular membrane protein VAMP2, dimeric pairs of the sensors could be used as in vivo ratiometric probes for metals. Thus, these engineered probes act as sensitive transition metal ion-responsive genetically encoded probes that span the visible spectrum." @default.
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- W4235981932 date "2014-01-01" @default.
- W4235981932 modified "2023-10-16" @default.
- W4235981932 title "An Engineered Palette of Metal Ion Quenchable Fluorescent Proteins" @default.
- W4235981932 doi "https://doi.org/10.1016/j.bpj.2013.11.2352" @default.
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