FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4236021452> ?p ?o ?g. }

• W4236021452 abstract "Abstract Background: Glucosamine (GlcN) functions as a building block of the cartilage matrix and its multifaceted roles in promoting joint health has been extensively investigated. On the contrary, the role of GlcN in osteogenesis and bone tissue is poorly understood, mainly due to the lack of adequate experimental models. Consequently, the benefit of GlcN in bone disorders remains controversial. In order to broaden the pharmacological relevance and potential therapeutic/nutraceutic efficacy of GlcN, we investigated the effect of GlcN on human primary osteoclasts (hOCs) and osteoblasts (hOBs) that were grown with 2D traditional methods or co-cultured in a more complex culture system one step closer to the in vivo bone microenvironment, consisting in a three-dimensional (3D) dynamic system. Methods: Osteoclasts derived from peripheral blood monocytes (hMCs) of either healthy or osteoarthritis (OA) donors, were used and kept in culture with osteoclastogenic inducers. hMCs were also combined with hOBs in a 3D dynamic co‐culture system by using RCCS-4TM bioreactor in osteogenic medium without osteoclastogenic inducers. In this condition osteoclastogenesis was supported by hOBs and sizeable self assembling aggregates were obtained. The differentiated osteoclasts were evaluated by the tartrate-resistant acid phosphatase assay, osteogenic differentiation was monitored by analyzing mineral matrix deposition through Alizarin Red staining, and expression of osteogenic markers through RT-qPCR. The cells were treated with DONA® Crystalline Glucosamine Sulfate (i.e. the original GlcN sulfate product). Results: DONA® was effective in decreasing the hOCs differentiation and function. Osteoclasts from OA donors were more sensitive than those from healthy donors. At the same time, DONA® showed anabolic effects on osteoblasts both in 2D conventional cell culture and in osteoclasts/osteoblasts 3D dynamic co-culture system. Conclusions: Here we demonstrated the effectiveness of a 3D dynamic co-culture system to provide useful information on the spectrum of action of GlcN on bone microenvironment. This can pave the way to better define the potential applications of a compound such as GlcN which is positioned between pharmaceuticals and nutraceuticals. Therefore, based on our observations, we hypothesize that GlcN could have potential benefits either in the treatment of osteopenic diseases such as osteoporosis, or in the bone health maintenance." @default.
• W4236021452 created "2022-05-12" @default.
• W4236021452 creator A5013758426 @default.
• W4236021452 creator A5017778705 @default.
• W4236021452 creator A5057166678 @default.
• W4236021452 creator A5063959885 @default.
• W4236021452 creator A5080670556 @default.
• W4236021452 creator A5081243343 @default.
• W4236021452 date "2020-09-09" @default.
• W4236021452 modified "2023-09-24" @default.
• W4236021452 title "Human Osteoclasts/Osteoblasts 3D Dynamic Co-Culture System to Study the Beneficial Effects of Glucosamine on Bone Microenvironment" @default.
• W4236021452 doi "https://doi.org/10.21203/rs.3.rs-71570/v1" @default.
• W4236021452 hasPublicationYear "2020" @default.
• W4236021452 type Work @default.
• W4236021452 citedByCount "0" @default.
• W4236021452 crossrefType "posted-content" @default.
• W4236021452 hasAuthorship W4236021452A5013758426 @default.
• W4236021452 hasAuthorship W4236021452A5017778705 @default.
• W4236021452 hasAuthorship W4236021452A5057166678 @default.
• W4236021452 hasAuthorship W4236021452A5063959885 @default.
• W4236021452 hasAuthorship W4236021452A5080670556 @default.
• W4236021452 hasAuthorship W4236021452A5081243343 @default.
• W4236021452 hasBestOaLocation W42360214521 @default.
• W4236021452 hasConcept C106487976 @default.
• W4236021452 hasConcept C126322002 @default.
• W4236021452 hasConcept C180675623 @default.
• W4236021452 hasConcept C185592680 @default.
• W4236021452 hasConcept C202751555 @default.
• W4236021452 hasConcept C2776033226 @default.
• W4236021452 hasConcept C2778125326 @default.
• W4236021452 hasConcept C43617362 @default.
• W4236021452 hasConcept C55493867 @default.
• W4236021452 hasConcept C71924100 @default.
• W4236021452 hasConcept C86803240 @default.
• W4236021452 hasConcept C95444343 @default.
• W4236021452 hasConceptScore W4236021452C106487976 @default.
• W4236021452 hasConceptScore W4236021452C126322002 @default.
• W4236021452 hasConceptScore W4236021452C180675623 @default.
• W4236021452 hasConceptScore W4236021452C185592680 @default.
• W4236021452 hasConceptScore W4236021452C202751555 @default.
• W4236021452 hasConceptScore W4236021452C2776033226 @default.
• W4236021452 hasConceptScore W4236021452C2778125326 @default.
• W4236021452 hasConceptScore W4236021452C43617362 @default.
• W4236021452 hasConceptScore W4236021452C55493867 @default.
• W4236021452 hasConceptScore W4236021452C71924100 @default.
• W4236021452 hasConceptScore W4236021452C86803240 @default.
• W4236021452 hasConceptScore W4236021452C95444343 @default.
• W4236021452 hasLocation W42360214521 @default.
• W4236021452 hasLocation W42360214522 @default.
• W4236021452 hasLocation W42360214523 @default.
• W4236021452 hasOpenAccess W4236021452 @default.
• W4236021452 hasPrimaryLocation W42360214521 @default.
• W4236021452 hasRelatedWork W1992762511 @default.
• W4236021452 hasRelatedWork W2027215054 @default.
• W4236021452 hasRelatedWork W2043919306 @default.
• W4236021452 hasRelatedWork W2072466744 @default.
• W4236021452 hasRelatedWork W2357143159 @default.
• W4236021452 hasRelatedWork W2364754457 @default.
• W4236021452 hasRelatedWork W2366765470 @default.
• W4236021452 hasRelatedWork W4233365581 @default.
• W4236021452 hasRelatedWork W4236021452 @default.
• W4236021452 hasRelatedWork W4245356512 @default.
• W4236021452 isParatext "false" @default.
• W4236021452 isRetracted "false" @default.
• W4236021452 workType "article" @default.