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- W4236024954 abstract "A standard nanospray-liquid chromatography–tandem mass spectrometry system in a column switching setup for the absolute quantification of leucine-enkephalin was evaluated. Analytes were loaded on a C18 trapping column and back-flushed in the 75 μm analytical column. Quantification was performed with a triple quadrupole instrument. Validation results show that it is feasible, with a conventional nano-LC system in the column switching setup, to quantify peptides as low as 500 amol on column (50 pmol/L). Weighted linear regression analysis proves a good linearity in a dynamic range of almost three orders of magnitude. Nevertheless, robustness remains a key issue in nano-LC–MS/MS." @default.
- W4236024954 created "2022-05-12" @default.
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- W4236024954 date "2004-11-26" @default.
- W4236024954 modified "2023-10-17" @default.
- W4236024954 title "Evaluation of nano-liquid chromatography–tandem mass spectrometry in a column switching setup for the absolute quantification of peptides in the picomolar range" @default.
- W4236024954 doi "https://doi.org/10.1016/s0021-9673(04)01304-4" @default.
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