Matches in SemOpenAlex for { <https://semopenalex.org/work/W4236025605> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W4236025605 endingPage "735" @default.
- W4236025605 startingPage "726" @default.
- W4236025605 abstract "We describe a methodology which allows the introduction of a photoactivatable azido group at specific internal positions of any RNA in order to identify the neighboring elements of an interacting protein. The first step involves site-directed modification of the target RNA with an antisense oligodeoxyribonucleotide bearing, at its 3′ or 5′ phosphate, a 4-[-N -(2-chloroethyl)-N -methylamino]benzylmethylamino group. Position N7 of a guanine residue located in the close vicinity of the hybrid is the main target for alkylation. The antisense oligodeoxyribonucleotide is then removed by acidic pH treatment and a photoreactive reagent (2,4-dinitro-5-fluorophenylazide) is condensed to the modified nucleotide. This method was used to induce specific cross-links between Escherichia coli threonyl-tRNA synthetase and the leader region of threonyl-tRNA synthetase mRNA, which is involved in translational feedback regulation. Control experiments revealed that the modification affects neither the structure of the mRNA nor the interaction with the enzyme. More than 50% of the modified mRNA complexed with threonyl-tRNA synthetase can be cross-linked to the enzyme, depending on the nucleotide modified." @default.
- W4236025605 created "2022-05-12" @default.
- W4236025605 creator A5007837003 @default.
- W4236025605 creator A5011517204 @default.
- W4236025605 creator A5024980365 @default.
- W4236025605 creator A5042609914 @default.
- W4236025605 creator A5064083080 @default.
- W4236025605 creator A5065701072 @default.
- W4236025605 creator A5067408390 @default.
- W4236025605 date "1995-08-01" @default.
- W4236025605 modified "2023-09-25" @default.
- W4236025605 title "A Novel Approach to Introduce Site-Directed Specific Cross-Links Within RNA-Protein Complexes. Application to the Escherichia Coli Threonyl-tRNA Synthetase/Translational Operator Complex" @default.
- W4236025605 cites W1973438658 @default.
- W4236025605 cites W1975013620 @default.
- W4236025605 cites W1997237467 @default.
- W4236025605 cites W2013958637 @default.
- W4236025605 cites W2022896690 @default.
- W4236025605 cites W2023597538 @default.
- W4236025605 cites W2035728389 @default.
- W4236025605 cites W2073307682 @default.
- W4236025605 cites W2119507985 @default.
- W4236025605 cites W2151694828 @default.
- W4236025605 doi "https://doi.org/10.1111/j.1432-1033.1995.tb20754.x" @default.
- W4236025605 hasPublicationYear "1995" @default.
- W4236025605 type Work @default.
- W4236025605 citedByCount "0" @default.
- W4236025605 crossrefType "journal-article" @default.
- W4236025605 hasAuthorship W4236025605A5007837003 @default.
- W4236025605 hasAuthorship W4236025605A5011517204 @default.
- W4236025605 hasAuthorship W4236025605A5024980365 @default.
- W4236025605 hasAuthorship W4236025605A5042609914 @default.
- W4236025605 hasAuthorship W4236025605A5064083080 @default.
- W4236025605 hasAuthorship W4236025605A5065701072 @default.
- W4236025605 hasAuthorship W4236025605A5067408390 @default.
- W4236025605 hasBestOaLocation W42360256051 @default.
- W4236025605 hasConcept C104317684 @default.
- W4236025605 hasConcept C105580179 @default.
- W4236025605 hasConcept C153911025 @default.
- W4236025605 hasConcept C153957851 @default.
- W4236025605 hasConcept C181199279 @default.
- W4236025605 hasConcept C185592680 @default.
- W4236025605 hasConcept C2778301229 @default.
- W4236025605 hasConcept C2779315393 @default.
- W4236025605 hasConcept C512185932 @default.
- W4236025605 hasConcept C547475151 @default.
- W4236025605 hasConcept C55493867 @default.
- W4236025605 hasConcept C67705224 @default.
- W4236025605 hasConcept C86803240 @default.
- W4236025605 hasConceptScore W4236025605C104317684 @default.
- W4236025605 hasConceptScore W4236025605C105580179 @default.
- W4236025605 hasConceptScore W4236025605C153911025 @default.
- W4236025605 hasConceptScore W4236025605C153957851 @default.
- W4236025605 hasConceptScore W4236025605C181199279 @default.
- W4236025605 hasConceptScore W4236025605C185592680 @default.
- W4236025605 hasConceptScore W4236025605C2778301229 @default.
- W4236025605 hasConceptScore W4236025605C2779315393 @default.
- W4236025605 hasConceptScore W4236025605C512185932 @default.
- W4236025605 hasConceptScore W4236025605C547475151 @default.
- W4236025605 hasConceptScore W4236025605C55493867 @default.
- W4236025605 hasConceptScore W4236025605C67705224 @default.
- W4236025605 hasConceptScore W4236025605C86803240 @default.
- W4236025605 hasIssue "3" @default.
- W4236025605 hasLocation W42360256051 @default.
- W4236025605 hasOpenAccess W4236025605 @default.
- W4236025605 hasPrimaryLocation W42360256051 @default.
- W4236025605 hasRelatedWork W1550615727 @default.
- W4236025605 hasRelatedWork W1554422725 @default.
- W4236025605 hasRelatedWork W2000424976 @default.
- W4236025605 hasRelatedWork W2022438440 @default.
- W4236025605 hasRelatedWork W2027408265 @default.
- W4236025605 hasRelatedWork W2054430652 @default.
- W4236025605 hasRelatedWork W2056019752 @default.
- W4236025605 hasRelatedWork W2070388274 @default.
- W4236025605 hasRelatedWork W2921287511 @default.
- W4236025605 hasRelatedWork W40679281 @default.
- W4236025605 hasVolume "231" @default.
- W4236025605 isParatext "false" @default.
- W4236025605 isRetracted "false" @default.
- W4236025605 workType "article" @default.