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- W4236045744 abstract "The benzo[a]pyrene (BP)-derived 7-(benzo[a]pyren-6-yl)guanine (BP-6-N7Gua) depurinating one-electron oxidation adduct was identified in the urine extracts of coal-smoke-exposed humans for the first time. Urine samples were prepared by solid-phase extraction and reversed-phase high-performance liquid chromatography. Subsequently, the BP-6-N7Gua adduct was identified on-line with capillary electrophoresis - fluorescence line narrowing spectroscopy (CE-FLNS) at 4.2 K. The daily excretion of BP-6-N7Gua in human urine of individuals exposed to coal smoke was approximately 226 pmol per μmol of creatinine. Due to the high level of excretion we propose that BP-6-N7Gua adducts found in urine could serve as effective biomarkers for risk assessment of BP exposure. The results demonstrate that CE-FLNS allows for on-line separation and DNA adducts identification in complex fluid extracts." @default.
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- W4236045744 date "2000-03-01" @default.
- W4236045744 modified "2023-09-23" @default.
- W4236045744 title "On-line identification of depurinating DNA adducts in human urine by capillary electrophoresis — fluorescence line narrowing spectroscopy" @default.
- W4236045744 doi "https://doi.org/10.1002/(sici)1522-2683(20000301)21:4<799::aid-elps799>3.3.co;2-m" @default.
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