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- W4236047104 abstract "Small et al. devoted a significant part of their pioneering work in ion chromatography to the separation and determination of cations. It comprised a low-capacity cation exchanger and a suppressor column containing a strong basic anion exchange resin in the hydroxide form. As in anion analysis, the application of a suppressor system is not necessarily a prerequisite for the cation determination by means of conductivity detection. The separation of cations is determined by their different affinities toward the stationary phase. The isocratic separation between monoethanolamine and ammonium is generally difficult. Higher polyamines such as triamines and tetramines can be separated under similar chromatographic conditions on IonPac CS18. Depending on the protein, ion-exchange (IEX), size-exclusion (SEC), affinity (IMAC), hydrophobic interaction (HIC), and reversed-phase chromatography (RPLC) may be applied. Porous polymer monoliths are successfully used for the separation of biomolecules such as peptides and proteins for more than 10 years." @default.
- W4236047104 created "2022-05-12" @default.
- W4236047104 date "2016-06-06" @default.
- W4236047104 modified "2023-09-26" @default.
- W4236047104 title "Cation‐Exchange Chromatography ( <scp>HPIC</scp> )" @default.
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- W4236047104 doi "https://doi.org/10.1002/9783527651610.ch4b" @default.
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