Matches in SemOpenAlex for { <https://semopenalex.org/work/W4236060862> ?p ?o ?g. }
Showing items 1 to 60 of
60
with 100 items per page.
- W4236060862 endingPage "tw276" @default.
- W4236060862 startingPage "tw276" @default.
- W4236060862 abstract "Wnt signaling leads to the stabilization of β-catenin, which then acts as a transcriptional activator in concert with the transcription factor TCF/LEF. In skin, activation of this signaling pathway promotes hair follicle cell fate. DasGupta et al. used a transgenic mouse model expressing a COOH-terminally truncated version of β-catenin that lacked the DNA binding domain to study cell fate specification in mouse skin (ΔNΔCβ-catenin). (The construct also lacked the NH2-terminal region and so was stabilized and not subject to the normal β-catenin degradation pathway.) They found that this ΔNΔCβ-catenin acted as a dominant negative in the cells that form the hair follicle, converting these into an epidermal cell type and promoting the formation of a cyst instead of a hair follicle. Analysis of promoter activity using mice carrying a TCF/LEF-responsive reporter showed that in the mice expressing ΔNΔCβ-catenin, the precursor hair cells did not activate the TCF/LEF reporter. In a subset of cells closer to the skin surface, the ΔNΔCβ-catenin acted as a gain-of-function mutation with the formation of abnormal flowerlike structures in the epidermis that eventually formed hair cell tumors. The cells at the base of these structures had intense TCF/LEF reporter gene expression, suggesting activation of the Wnt pathway. Using cultured primary cells from the transgenic animals, the authors found that in keratinocytes stimulated to differentiate by the addition of calcium, endogenous β-catenin was stabilized and localized to the nucleus in the cells, consistent with a positive effect of ΔNΔCβ-catenin on Wnt signaling. In the epidermal cysts formed instead of normal hair follicles, the ratio of ΔNΔCβ-catenin to endogenous β-catenin was high, consistent with the inhibitory effect of ΔNΔCβ-catenin on Wnt signaling. Thus, β-catenin signaling is different in these cells, possibly reflecting differences in the abundance of interacting factors.R. DasGupta, H. Rhee, E. Fuchs, A developmental conundrum: a stabilized form of β-catenin lacking the transcriptional activation domain triggers features of hair cell fate in epidermal cells and epidermal cell fate in hair follicle cells. J. Cell. Biol. 158, 331-344 (2002). [Abstract] [Full Text]" @default.
- W4236060862 created "2022-05-12" @default.
- W4236060862 date "2002-07-30" @default.
- W4236060862 modified "2023-10-18" @default.
- W4236060862 title "One Transgene: Two Outcomes" @default.
- W4236060862 doi "https://doi.org/10.1126/scisignal.1432002tw276" @default.
- W4236060862 hasPublicationYear "2002" @default.
- W4236060862 type Work @default.
- W4236060862 citedByCount "0" @default.
- W4236060862 crossrefType "journal-article" @default.
- W4236060862 hasConcept C102230213 @default.
- W4236060862 hasConcept C104317684 @default.
- W4236060862 hasConcept C105702510 @default.
- W4236060862 hasConcept C137620995 @default.
- W4236060862 hasConcept C150194340 @default.
- W4236060862 hasConcept C153911025 @default.
- W4236060862 hasConcept C161733203 @default.
- W4236060862 hasConcept C2776458125 @default.
- W4236060862 hasConcept C2777216303 @default.
- W4236060862 hasConcept C30145163 @default.
- W4236060862 hasConcept C54355233 @default.
- W4236060862 hasConcept C62478195 @default.
- W4236060862 hasConcept C86339819 @default.
- W4236060862 hasConcept C86803240 @default.
- W4236060862 hasConcept C95444343 @default.
- W4236060862 hasConceptScore W4236060862C102230213 @default.
- W4236060862 hasConceptScore W4236060862C104317684 @default.
- W4236060862 hasConceptScore W4236060862C105702510 @default.
- W4236060862 hasConceptScore W4236060862C137620995 @default.
- W4236060862 hasConceptScore W4236060862C150194340 @default.
- W4236060862 hasConceptScore W4236060862C153911025 @default.
- W4236060862 hasConceptScore W4236060862C161733203 @default.
- W4236060862 hasConceptScore W4236060862C2776458125 @default.
- W4236060862 hasConceptScore W4236060862C2777216303 @default.
- W4236060862 hasConceptScore W4236060862C30145163 @default.
- W4236060862 hasConceptScore W4236060862C54355233 @default.
- W4236060862 hasConceptScore W4236060862C62478195 @default.
- W4236060862 hasConceptScore W4236060862C86339819 @default.
- W4236060862 hasConceptScore W4236060862C86803240 @default.
- W4236060862 hasConceptScore W4236060862C95444343 @default.
- W4236060862 hasIssue "143" @default.
- W4236060862 hasLocation W42360608621 @default.
- W4236060862 hasOpenAccess W4236060862 @default.
- W4236060862 hasPrimaryLocation W42360608621 @default.
- W4236060862 hasRelatedWork W1577141571 @default.
- W4236060862 hasRelatedWork W1996861298 @default.
- W4236060862 hasRelatedWork W2011842366 @default.
- W4236060862 hasRelatedWork W2034986957 @default.
- W4236060862 hasRelatedWork W2041577331 @default.
- W4236060862 hasRelatedWork W2083489441 @default.
- W4236060862 hasRelatedWork W2094394048 @default.
- W4236060862 hasRelatedWork W2154964320 @default.
- W4236060862 hasRelatedWork W2169586407 @default.
- W4236060862 hasRelatedWork W2913359100 @default.
- W4236060862 hasVolume "2002" @default.
- W4236060862 isParatext "false" @default.
- W4236060862 isRetracted "false" @default.
- W4236060862 workType "article" @default.