Matches in SemOpenAlex for { <https://semopenalex.org/work/W4236063138> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W4236063138 endingPage "403" @default.
- W4236063138 startingPage "400" @default.
- W4236063138 abstract "Objective. To determine functional anti-LPS specific serum bactericidal antibody (SBA) and opsonophagocytic killing antibody (OPKA) activities in mice immunized with a pentavalent candidate vaccine against Shigella flexneri 1b, 2a, 3a, 6, Y (PF). Materials and Methods. (CBA x C57 B1/6) F1 mice were immunized with a PF. 14 days after the reimmunization, serum samples were collected and the level of specific IgG in them was determined by ELISA. The sera of mice immunized with individual modified lipopolysaccharides (mLPS), which are part of PF, were used for positive control. Serum from intact mice served as a negative control. The functional activity of serum antibodies was determined by the methods of SBA and OPKA assay. The result was evaluated by the percentage of bacteria killed. Results. In all experimental groups of mice, an increase in the titer of specific IgG is observed (p < 0.05). The endpoint titer (ET) of anti-LPS S. flexneri 1b, 2a, 3a, 6, Y antibodies in the group of mice immunized with PF does not significantly differ from ET in the groups after immunizations with individual mLPS. At the same time, the indicators in the experimental groups are about 16 times higher than in the control. We determined a functional activity of S. flexneri-specific SBA and OPRA in mice immunized with PF. The rate of SBA killing was 54%, 66%, 35%, 60%, 60% for S. flexneri 1b, 2a, 3a, 6, Y serotypes, respectively. When OPKA killing in groups of immunized mice are 37%, 55%, 27%, 56%, 53% for S. flexneri 1b, 2a, 3a, 6, Y serotypes, respectively. Conclusions. PF induces the production of specific anti-LPS IgG comparable to its individual components. The sera from PF immunized mice contain functional antibodies. Serum bactericidal and opsonophagocytic assay are effective for use in a mouse model." @default.
- W4236063138 created "2022-05-12" @default.
- W4236063138 creator A5072189540 @default.
- W4236063138 date "2021-01-01" @default.
- W4236063138 modified "2023-09-30" @default.
- W4236063138 title "Functional and antigenspecific serum antibodies in mice after immunization with a candidate vaccine against Shigella flexneri 1b, 2a, 3a, 6, Y" @default.
- W4236063138 cites W1627569562 @default.
- W4236063138 cites W1970216382 @default.
- W4236063138 cites W2050192744 @default.
- W4236063138 cites W2115350923 @default.
- W4236063138 cites W2115734061 @default.
- W4236063138 cites W2123227667 @default.
- W4236063138 cites W2560777220 @default.
- W4236063138 cites W2908796561 @default.
- W4236063138 cites W2951541824 @default.
- W4236063138 cites W4211093840 @default.
- W4236063138 cites W4251095467 @default.
- W4236063138 doi "https://doi.org/10.36488/cmac.2021.4.400-403" @default.
- W4236063138 hasPublicationYear "2021" @default.
- W4236063138 type Work @default.
- W4236063138 citedByCount "0" @default.
- W4236063138 crossrefType "journal-article" @default.
- W4236063138 hasAuthorship W4236063138A5072189540 @default.
- W4236063138 hasBestOaLocation W42360631381 @default.
- W4236063138 hasConcept C10389963 @default.
- W4236063138 hasConcept C104317684 @default.
- W4236063138 hasConcept C159047783 @default.
- W4236063138 hasConcept C159654299 @default.
- W4236063138 hasConcept C203014093 @default.
- W4236063138 hasConcept C22889606 @default.
- W4236063138 hasConcept C2780801004 @default.
- W4236063138 hasConcept C2781217973 @default.
- W4236063138 hasConcept C32611913 @default.
- W4236063138 hasConcept C547475151 @default.
- W4236063138 hasConcept C55493867 @default.
- W4236063138 hasConcept C71924100 @default.
- W4236063138 hasConcept C86803240 @default.
- W4236063138 hasConcept C89423630 @default.
- W4236063138 hasConceptScore W4236063138C10389963 @default.
- W4236063138 hasConceptScore W4236063138C104317684 @default.
- W4236063138 hasConceptScore W4236063138C159047783 @default.
- W4236063138 hasConceptScore W4236063138C159654299 @default.
- W4236063138 hasConceptScore W4236063138C203014093 @default.
- W4236063138 hasConceptScore W4236063138C22889606 @default.
- W4236063138 hasConceptScore W4236063138C2780801004 @default.
- W4236063138 hasConceptScore W4236063138C2781217973 @default.
- W4236063138 hasConceptScore W4236063138C32611913 @default.
- W4236063138 hasConceptScore W4236063138C547475151 @default.
- W4236063138 hasConceptScore W4236063138C55493867 @default.
- W4236063138 hasConceptScore W4236063138C71924100 @default.
- W4236063138 hasConceptScore W4236063138C86803240 @default.
- W4236063138 hasConceptScore W4236063138C89423630 @default.
- W4236063138 hasIssue "4" @default.
- W4236063138 hasLocation W42360631381 @default.
- W4236063138 hasLocation W42360631382 @default.
- W4236063138 hasOpenAccess W4236063138 @default.
- W4236063138 hasPrimaryLocation W42360631381 @default.
- W4236063138 hasRelatedWork W1972745941 @default.
- W4236063138 hasRelatedWork W1984647379 @default.
- W4236063138 hasRelatedWork W2374934415 @default.
- W4236063138 hasRelatedWork W2375252691 @default.
- W4236063138 hasRelatedWork W2384157809 @default.
- W4236063138 hasRelatedWork W2388057270 @default.
- W4236063138 hasRelatedWork W2391993932 @default.
- W4236063138 hasRelatedWork W2414181163 @default.
- W4236063138 hasRelatedWork W4307264905 @default.
- W4236063138 hasRelatedWork W1840551154 @default.
- W4236063138 hasVolume "23" @default.
- W4236063138 isParatext "false" @default.
- W4236063138 isRetracted "false" @default.
- W4236063138 workType "article" @default.