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- W4236733500 abstract "Abstract The present work shows the implementation of the Response Surface Methodology (RSM), fed by an experimental Central Composite Design (CCD) to find the conditions that allow maximizing the inhibition of the microorganism Staphylococcus aureus with nanoparticles of TiO 2 silanized with 3-Aminopropyltriethoxysilane (APTES) and doped with Ag. In addition, Poly(lactic) acid composites were prepared with these Ag/TiO 2 nanoparticles with the aim to confer their antimicrobial effect. The independent variables considered were pH, AgNO 3 /TiO 2 ratio (% w/w), and TiO 2 nanoparticles concentration (g/250 mL), and as the variable of response, the length of the diameter of the halo or zone of inhibition presented by the microorganism (mm). Statistical analysis found that maximization of S. aureus inhibition occurs at intermediate levels with a value of 10 for pH and 5 g of TiO 2 solids, while for the concentration of AgNO 3 high levels are required, greater than 10% w/w. Likewise, the statistical significance was determined using the Student's t-test and the p-value; it was found that the significant effect corresponds to the concentration of AgNO 3 , so a second experimental CCD design equirradial with two factors was considered, estimating AgNO 3 concentration and TiO 2 amount, the pH at constant 10 value. The second experimental design indicated that maximization in S. aureus inhibition occurs at an AgNO 3 concentration between 20-25% w/w with high amounts of TiO 2 solids (7-8 g), with a resulting zone of inhibition between 26-28 mm. The quadratic model obtained, which represents the relationship between the length of the zone of inhibition with the variables considered, shows an adjustment of experimental data with a coefficient of determination (R 2 ) of 0.82." @default.
- W4236733500 created "2022-05-12" @default.
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- W4236733500 date "2021-08-10" @default.
- W4236733500 modified "2023-10-16" @default.
- W4236733500 title "Application of the response surface methodology for the evaluation of Staphylococcus aureus inhibition with Ag/TiO2 nanoparticles" @default.
- W4236733500 doi "https://doi.org/10.21203/rs.3.rs-617251/v1" @default.
- W4236733500 hasPublicationYear "2021" @default.
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