Matches in SemOpenAlex for { <https://semopenalex.org/work/W4236978444> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W4236978444 abstract "Abstract Various methods have been developed for rapid and high throughput full genome sequencing of SARS-CoV-2. Here, we described a protocol for targeted multiplex full genome sequencing of SARS-CoV-2 genomic RNA directly extracted from human nasopharyngeal swabs using the Ion Personal Genome Machine (PGM). This protocol involves concomitant amplification of 237 gene fragments encompassing the SARS-CoV-2 genome to increase the abundance and yield of viral specific sequencing reads. Five complete and one near-complete genome sequences of SARS-CoV-2 were generated with a single Ion PGM sequencing run. The sequence coverage analysis revealed two amplicons (positions 13751-13965 and 23941-24106), which consistently gave low sequencing read coverage in all isolates except 4Apr20-64-Hu. We analyzed the potential primer binding sites within these low covered regions and noted that the 4Apr20-64-Hu possess C at positions 13730 and 23929, whereas the other isolates possess T at these positions. The genetic variations observed suggest that the naturally occurring genome variations present in the actively circulating SARS-CoV-2 strains affected the performance of the target enrichment panel of the Ion AmpliSeq™ SARS‑CoV‑2 Research Panel. The possible impact of other genetic sequence variations warrants further investigation, and an improved version of the Ion AmpliSeq™ SARS‑CoV‑2 Research Panel, hence, should be considered." @default.
- W4236978444 created "2022-05-12" @default.
- W4236978444 creator A5001300487 @default.
- W4236978444 creator A5003116521 @default.
- W4236978444 creator A5018901443 @default.
- W4236978444 creator A5019082416 @default.
- W4236978444 creator A5024766546 @default.
- W4236978444 creator A5025393367 @default.
- W4236978444 creator A5026821024 @default.
- W4236978444 creator A5027448959 @default.
- W4236978444 creator A5028234391 @default.
- W4236978444 creator A5048994912 @default.
- W4236978444 creator A5055991519 @default.
- W4236978444 creator A5058900492 @default.
- W4236978444 creator A5060463269 @default.
- W4236978444 creator A5081218230 @default.
- W4236978444 creator A5084155517 @default.
- W4236978444 date "2021-02-08" @default.
- W4236978444 modified "2023-09-28" @default.
- W4236978444 title "Multiplex Sequencing of SARS-Cov-2 genome directly from clinical samples using the Ion Personal Genome Machine (PGM)" @default.
- W4236978444 doi "https://doi.org/10.21203/rs.3.rs-141611/v1" @default.
- W4236978444 hasPublicationYear "2021" @default.
- W4236978444 type Work @default.
- W4236978444 citedByCount "0" @default.
- W4236978444 crossrefType "posted-content" @default.
- W4236978444 hasAuthorship W4236978444A5001300487 @default.
- W4236978444 hasAuthorship W4236978444A5003116521 @default.
- W4236978444 hasAuthorship W4236978444A5018901443 @default.
- W4236978444 hasAuthorship W4236978444A5019082416 @default.
- W4236978444 hasAuthorship W4236978444A5024766546 @default.
- W4236978444 hasAuthorship W4236978444A5025393367 @default.
- W4236978444 hasAuthorship W4236978444A5026821024 @default.
- W4236978444 hasAuthorship W4236978444A5027448959 @default.
- W4236978444 hasAuthorship W4236978444A5028234391 @default.
- W4236978444 hasAuthorship W4236978444A5048994912 @default.
- W4236978444 hasAuthorship W4236978444A5055991519 @default.
- W4236978444 hasAuthorship W4236978444A5058900492 @default.
- W4236978444 hasAuthorship W4236978444A5060463269 @default.
- W4236978444 hasAuthorship W4236978444A5081218230 @default.
- W4236978444 hasAuthorship W4236978444A5084155517 @default.
- W4236978444 hasBestOaLocation W42369784441 @default.
- W4236978444 hasConcept C104317684 @default.
- W4236978444 hasConcept C141231307 @default.
- W4236978444 hasConcept C164928082 @default.
- W4236978444 hasConcept C205270622 @default.
- W4236978444 hasConcept C24103923 @default.
- W4236978444 hasConcept C24432333 @default.
- W4236978444 hasConcept C2781188995 @default.
- W4236978444 hasConcept C49105822 @default.
- W4236978444 hasConcept C51679486 @default.
- W4236978444 hasConcept C54355233 @default.
- W4236978444 hasConcept C70721500 @default.
- W4236978444 hasConcept C8185291 @default.
- W4236978444 hasConcept C86803240 @default.
- W4236978444 hasConceptScore W4236978444C104317684 @default.
- W4236978444 hasConceptScore W4236978444C141231307 @default.
- W4236978444 hasConceptScore W4236978444C164928082 @default.
- W4236978444 hasConceptScore W4236978444C205270622 @default.
- W4236978444 hasConceptScore W4236978444C24103923 @default.
- W4236978444 hasConceptScore W4236978444C24432333 @default.
- W4236978444 hasConceptScore W4236978444C2781188995 @default.
- W4236978444 hasConceptScore W4236978444C49105822 @default.
- W4236978444 hasConceptScore W4236978444C51679486 @default.
- W4236978444 hasConceptScore W4236978444C54355233 @default.
- W4236978444 hasConceptScore W4236978444C70721500 @default.
- W4236978444 hasConceptScore W4236978444C8185291 @default.
- W4236978444 hasConceptScore W4236978444C86803240 @default.
- W4236978444 hasLocation W42369784441 @default.
- W4236978444 hasOpenAccess W4236978444 @default.
- W4236978444 hasPrimaryLocation W42369784441 @default.
- W4236978444 hasRelatedWork W1978155433 @default.
- W4236978444 hasRelatedWork W2016680388 @default.
- W4236978444 hasRelatedWork W2047669659 @default.
- W4236978444 hasRelatedWork W2151223725 @default.
- W4236978444 hasRelatedWork W2522810657 @default.
- W4236978444 hasRelatedWork W2734730316 @default.
- W4236978444 hasRelatedWork W2903885984 @default.
- W4236978444 hasRelatedWork W3208139257 @default.
- W4236978444 hasRelatedWork W4236978444 @default.
- W4236978444 hasRelatedWork W2409976444 @default.
- W4236978444 isParatext "false" @default.
- W4236978444 isRetracted "false" @default.
- W4236978444 workType "article" @default.