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- W4237510010 abstract "The objective of this study was to assess the performance of a technique (S. PneumoStrip test) based on PCR followed by reverse strip hybridisation for the detection of Streptococcus pneumoniae serotypes directly in blood culture vials.One hundred and ten (110) pairs of isolated strains and their corresponding original blood cultures vials were studied in parallel. Pure isolated strains were conventionally serotyped using latex agglutination and the Quellung reaction. The S. PneumoStrip test was carried out directly in the original blood culture samples.In 102 cases (92.7%), results of the serotype obtained by Quellung coincided with their corresponding original blood cultures typed by S. PneumoStrip.S. PneumoStrip test is a good alternative technique for direct pneumococcal serotyping in blood culture clinical samples.El objetivo de este estudio fue evaluar el rendimiento de una técnica de PCR seguida de hibridación reversa (S. PneumoStrip test) para su aplicación directa en muestras de frasco de hemocultivo.Se estudiaron 110 cepas aisladas en sangre y sus correspondientes muestras de frasco de hemocultivo. Las cepas fueron serotipadas convencionalmente mediante aglutinación por látex y reacción de Quellung. El test de S. PneumoStrip se realizó directamente en las muestras originales de hemocultivo.En 102 casos (92,7%) el serotipado por Quellung coincidió con el obtenido directamente en su original frasco de hemocultivo.S. PneumoStrip constituye una buena alternativa para el serotipado directo en muestras de frasco de hemocultivo." @default.
- W4237510010 created "2022-05-12" @default.
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- W4237510010 date "2020-03-01" @default.
- W4237510010 modified "2023-10-16" @default.
- W4237510010 title "Lung nodules and retinal lesions in an immunocompromised patient" @default.
- W4237510010 doi "https://doi.org/10.1016/j.eimce.2019.07.005" @default.
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