Matches in SemOpenAlex for { <https://semopenalex.org/work/W4238100488> ?p ?o ?g. }
Showing items 1 to 56 of
56
with 100 items per page.
- W4238100488 endingPage "358" @default.
- W4238100488 startingPage "353" @default.
- W4238100488 abstract "Zinc protoporphyrin IX (ZnPP) inhibits production of carbon monoxide (CO) but not nitric oxide (NO) synthase activity in vitro, therefore, it has been used to discriminate the effects of these activators of cyclic GMP production. However, intracellular enzyme activity is supported by endogenous substrates and cofactors, therefore, in vitro experiments with an optimal supply of these substances do not necessarily represent the intracellular circumstances. The present study was conducted to examine whether ZnPP affects NO production in rat cerebellar slices. Incubation with 1-aminocyclopentane-trans-1,3-dicarboxylic acid (ACPD) increased the conversion of [3H]-l-arginine into [3H]-citrulline in the slices. ZnPP at 1 μM, not only suppressed the ACPD-dependent production of [3H]-citrulline but also reduced the basal [3H]-citrulline level, accumulation of [3H]-l-arginine and ATP content in the slices, but ZnPP at 1 μM did not affect the catalytic activity of partially purified NO synthases in vitro. In contrast, a selective inhibitor of NO synthase, Nω-nitro-l-arginine, inhibited ACPD-dependent [3H]-citrulline production without affecting the basal [3H]-citrulline level or [3H]-l-arginine accumulation. These results indicate that ZnPP suppresses NO production through a loss of its precursor, even at concentrations exhibiting no inhibition of the catalytic activity of NO synthase. It is concluded that ACPD activates NO synthase in the slice and that ZnPP cannot discriminate the roles of CO from NO in cyclic GMP production in brain slices." @default.
- W4238100488 created "2022-05-12" @default.
- W4238100488 creator A5085139457 @default.
- W4238100488 date "1996-07-23" @default.
- W4238100488 modified "2023-10-03" @default.
- W4238100488 title "Zinc protoporphyrin IX suppresses nitric oxide production through a loss of ?-arginine in rat cerebellar slices" @default.
- W4238100488 doi "https://doi.org/10.1016/s0168-0102(96)01057-7" @default.
- W4238100488 hasPublicationYear "1996" @default.
- W4238100488 type Work @default.
- W4238100488 citedByCount "2" @default.
- W4238100488 crossrefType "journal-article" @default.
- W4238100488 hasAuthorship W4238100488A5085139457 @default.
- W4238100488 hasConcept C178790620 @default.
- W4238100488 hasConcept C181199279 @default.
- W4238100488 hasConcept C185592680 @default.
- W4238100488 hasConcept C2776217839 @default.
- W4238100488 hasConcept C2777468819 @default.
- W4238100488 hasConcept C2777622882 @default.
- W4238100488 hasConcept C2779129087 @default.
- W4238100488 hasConcept C2781447869 @default.
- W4238100488 hasConcept C515207424 @default.
- W4238100488 hasConcept C519581460 @default.
- W4238100488 hasConcept C55493867 @default.
- W4238100488 hasConcept C79879829 @default.
- W4238100488 hasConceptScore W4238100488C178790620 @default.
- W4238100488 hasConceptScore W4238100488C181199279 @default.
- W4238100488 hasConceptScore W4238100488C185592680 @default.
- W4238100488 hasConceptScore W4238100488C2776217839 @default.
- W4238100488 hasConceptScore W4238100488C2777468819 @default.
- W4238100488 hasConceptScore W4238100488C2777622882 @default.
- W4238100488 hasConceptScore W4238100488C2779129087 @default.
- W4238100488 hasConceptScore W4238100488C2781447869 @default.
- W4238100488 hasConceptScore W4238100488C515207424 @default.
- W4238100488 hasConceptScore W4238100488C519581460 @default.
- W4238100488 hasConceptScore W4238100488C55493867 @default.
- W4238100488 hasConceptScore W4238100488C79879829 @default.
- W4238100488 hasIssue "4" @default.
- W4238100488 hasLocation W42381004881 @default.
- W4238100488 hasOpenAccess W4238100488 @default.
- W4238100488 hasPrimaryLocation W42381004881 @default.
- W4238100488 hasRelatedWork W2042748276 @default.
- W4238100488 hasRelatedWork W2047346085 @default.
- W4238100488 hasRelatedWork W2055574143 @default.
- W4238100488 hasRelatedWork W2080330601 @default.
- W4238100488 hasRelatedWork W2090957628 @default.
- W4238100488 hasRelatedWork W2093996623 @default.
- W4238100488 hasRelatedWork W2094931296 @default.
- W4238100488 hasRelatedWork W2167008296 @default.
- W4238100488 hasRelatedWork W2949423999 @default.
- W4238100488 hasRelatedWork W2188027336 @default.
- W4238100488 hasVolume "25" @default.
- W4238100488 isParatext "false" @default.
- W4238100488 isRetracted "false" @default.
- W4238100488 workType "article" @default.