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• W4238236906 abstract "A simple modification to the optical configuration used for fluorescence photoactivation localization microscopy (FPALM) allows the fluorescence anisotropies of each individual molecule in a nanoscale image to be measured. The method was used to obtain position and orientation information for fluorescently labeled actin or hemagglutinin molecules in fixed fibroblasts. Knowledge of the orientation of molecules within biological structures is crucial to understanding the mechanisms of cell function. We present a method to image simultaneously the positions and fluorescence anisotropies of large numbers of single molecules with nanometer lateral resolution within a sample. Based on a simple modification of fluorescence photoactivation localization microscopy (FPALM), polarization (P)-FPALM does not compromise speed or sensitivity. We show results for mouse fibroblasts expressing Dendra2-actin or Dendra2-hemagglutinin." @default.
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• W4238236906 date "2008-11-16" @default.
• W4238236906 modified "2023-10-10" @default.
• W4238236906 title "Nanoscale imaging of molecular positions and anisotropies" @default.
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• W4238236906 doi "https://doi.org/10.1038/nmeth.1271" @default.
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