Matches in SemOpenAlex for { <https://semopenalex.org/work/W4238261107> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W4238261107 abstract "Abstract Background Gallbladder cancer (GBC) accounts for 85–90% malignancies of the biliary tree worldwide. Considerable evidence has demonstrated that dysregulation of lncRNAs are involved in the progression of cancer. LncRNA PVT1 has been reported to play important roles in various cancers, but the role of PVT1 in gallbladder cancer remains unknown. Methods QRT-PCR was used to assess the expression of genes in different tissues or cells. Knockdown or overexpression of PVT1 combined with in vitro and in vivo assays were conducted to determine the effect of PVT1 on the GBC cells proliferation. We also conducted microarray analysis to screen out the miRNA that was regulated by PVT1. BSP was used to detect the methylation status of miR-18b-5p DNA promoter. RIP, ChIP, Co-IP and luciferase reporter assays were performed to validate the association between genes or proteins. Results We found that PVT1 was upregulated in GBC tissues and cells, and its upregulation was related with poor prognosis in GBC patients. PVT1 promoted GBC cells proliferation and increased tumorigenicity in nude mice. Molecular experiments demonstrated that PVT1 recruited DNMT1 via EZH2 to the miR-18b-5p DNA promoter and suppressed the transcription of miR-18b-5p through DNA methylation. Moreover, HIF1A was proved to be the downstream target gene of miR-18b-5p and PVT1 regulated GBC cell proliferation via HIF1A. Conclusions Our studies clarified the PVT1/ miR-18b-5p/ HIF1A regulation axis and indicated that PVT1 could be a potential therapeutic target for GBC." @default.
- W4238261107 created "2022-05-12" @default.
- W4238261107 creator A5036111054 @default.
- W4238261107 creator A5040623969 @default.
- W4238261107 creator A5043424443 @default.
- W4238261107 creator A5045901990 @default.
- W4238261107 creator A5059373453 @default.
- W4238261107 creator A5078893282 @default.
- W4238261107 date "2020-06-18" @default.
- W4238261107 modified "2023-10-14" @default.
- W4238261107 title "Long Noncoding RNA PVT1 promoted Gallbladder Cancer proliferation Byepigenetically Suppressing mIR-18b-5p via DNA Methylation" @default.
- W4238261107 doi "https://doi.org/10.21203/rs.3.rs-35981/v1" @default.
- W4238261107 hasPublicationYear "2020" @default.
- W4238261107 type Work @default.
- W4238261107 citedByCount "0" @default.
- W4238261107 crossrefType "posted-content" @default.
- W4238261107 hasAuthorship W4238261107A5036111054 @default.
- W4238261107 hasAuthorship W4238261107A5040623969 @default.
- W4238261107 hasAuthorship W4238261107A5043424443 @default.
- W4238261107 hasAuthorship W4238261107A5045901990 @default.
- W4238261107 hasAuthorship W4238261107A5059373453 @default.
- W4238261107 hasAuthorship W4238261107A5078893282 @default.
- W4238261107 hasBestOaLocation W42382611071 @default.
- W4238261107 hasConcept C104317684 @default.
- W4238261107 hasConcept C121608353 @default.
- W4238261107 hasConcept C127561419 @default.
- W4238261107 hasConcept C145059251 @default.
- W4238261107 hasConcept C150194340 @default.
- W4238261107 hasConcept C153911025 @default.
- W4238261107 hasConcept C173396325 @default.
- W4238261107 hasConcept C190727270 @default.
- W4238261107 hasConcept C2776534502 @default.
- W4238261107 hasConcept C2777394393 @default.
- W4238261107 hasConcept C33288867 @default.
- W4238261107 hasConcept C502942594 @default.
- W4238261107 hasConcept C54355233 @default.
- W4238261107 hasConcept C62203573 @default.
- W4238261107 hasConcept C86803240 @default.
- W4238261107 hasConceptScore W4238261107C104317684 @default.
- W4238261107 hasConceptScore W4238261107C121608353 @default.
- W4238261107 hasConceptScore W4238261107C127561419 @default.
- W4238261107 hasConceptScore W4238261107C145059251 @default.
- W4238261107 hasConceptScore W4238261107C150194340 @default.
- W4238261107 hasConceptScore W4238261107C153911025 @default.
- W4238261107 hasConceptScore W4238261107C173396325 @default.
- W4238261107 hasConceptScore W4238261107C190727270 @default.
- W4238261107 hasConceptScore W4238261107C2776534502 @default.
- W4238261107 hasConceptScore W4238261107C2777394393 @default.
- W4238261107 hasConceptScore W4238261107C33288867 @default.
- W4238261107 hasConceptScore W4238261107C502942594 @default.
- W4238261107 hasConceptScore W4238261107C54355233 @default.
- W4238261107 hasConceptScore W4238261107C62203573 @default.
- W4238261107 hasConceptScore W4238261107C86803240 @default.
- W4238261107 hasLocation W42382611071 @default.
- W4238261107 hasOpenAccess W4238261107 @default.
- W4238261107 hasPrimaryLocation W42382611071 @default.
- W4238261107 hasRelatedWork W14265183 @default.
- W4238261107 hasRelatedWork W16724221 @default.
- W4238261107 hasRelatedWork W18529857 @default.
- W4238261107 hasRelatedWork W26504633 @default.
- W4238261107 hasRelatedWork W29852207 @default.
- W4238261107 hasRelatedWork W37732940 @default.
- W4238261107 hasRelatedWork W4520284 @default.
- W4238261107 hasRelatedWork W5155421 @default.
- W4238261107 hasRelatedWork W7813389 @default.
- W4238261107 hasRelatedWork W30234032 @default.
- W4238261107 isParatext "false" @default.
- W4238261107 isRetracted "false" @default.
- W4238261107 workType "article" @default.