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- W4238271839 abstract "Treatment of Fusarium oxysporum with 5-azacytidine, a potent inhibitor of DNA methylation, induced nonpathogenic mutants. Analysis of the protein expression pattern by two-dimensional gel electrophoresis revealed a protein that is present in yeast-form cells of the mutants but absent in those of the wild-type strain. N-terminal amino acid analysis indicated that this protein is identical to a region of a polypeptide encoded by a cDNA clone, sti35, previously identified as a heat shock gene in F. oxysporum. A genomic clone for sti35 was isolated and sequence analysis revealed an intron and two heat shock elements upstream of sti35. The analysis also revealed the presence of a leader sequence composed of 27 amino acid residues, which shares a common amino acid composition with leader sequences of the proteins located in the mitochondrial matrix. Different expression patterns of sti35 in the mutants and wild-type strain were demonstrated." @default.
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- W4238271839 date "2000-01-01" @default.
- W4238271839 modified "2023-09-26" @default.
- W4238271839 title "Detection and Cloning of the Gene Encoding a Protein Produced by Nonpathogenic Mutants of Fusarium oxysporum." @default.
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- W4238271839 doi "https://doi.org/10.1263/jbb.90.302" @default.
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