Matches in SemOpenAlex for { <https://semopenalex.org/work/W4238465239> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W4238465239 endingPage "2109" @default.
- W4238465239 startingPage "2100" @default.
- W4238465239 abstract "Treatment of normal cartilage with transforming growth factor beta (TGFbeta) can increase the synthesis of collagenase 3 by chondrocytes and mimic the in situ distribution of this enzyme in osteoarthritic (OA) cartilage, which occurs predominantly in the deep zone. In this study, we examined the elements of the TGFbeta system that are potentially relevant to this effect.TGFbeta1 and TGFbeta2 levels in cultured cartilage explants were determined by enzyme-linked immunosorbent assay (ELISA). OA cartilage explants were treated with small latent TGFbeta1 complex in the presence of various inhibitors, and collagenase 3 levels were determined by ELISA. The inhibitors were against serine proteases, plasmin, cathepsins, furin, and a neutralizing antibody against the mannose-6 phosphate/ insulin-like growth factor 2 receptor (M6P/IGF-2R). Small latent TGFbeta1, TGFbeta receptor types I, II, and III (TGFbetaRI, RII, and RIII), M6P/IGF-2R, and furin were immunolocalized in cartilage.Our data showed that latent TGFbeta1 is the major isoform that is synthesized; levels of 17.2 +/-1.7 pg/mg and 1.1 +/- 0.3 pg/mg tissue wet weight (mean +/- SEM) were found for total TGFbeta1 and TGFbeta2, respectively, in OA cartilage. A general serine protease inhibitor abrogated activation of both endogenous and exogenous small latent TGFbeta1. Plasmin and furin inhibitors and anti-M6P/IGF-2R reduced the levels of exogenous small latent TGFbeta1 complex-induced collagenase 3 by 33%, 95%, and 76%, respectively, but the cathepsin inhibitor had no effect. Immunolocalization of the small latent TGFbeta1 complex as well as of TGFbetaRI and RII revealed a statistically significant increase in the chondrocyte score in only the deep zone of OA cartilage. The M6P/IGF-2R level was significantly higher in OA cartilage in both the superficial and deep zones. Furin was found in normal cartilage exclusively in the superficial zone, whereas in OA cartilage, a level similar to that in normal cartilage was found in the superficial zone, but a significantly higher cell score (mean +/- SEM 23.6 +/- 4.7%) was registered in the deep zone.The mechanisms of TGFbeta activation/ activity with regard to collagenase 3 modulation in cartilage appear to be controlled by furin convertase with or without M6P/IGF-2R. These factors and the small latent TGFbeta complex are increased in the deep zone of OA cartilage, corresponding to the preferential site of collagenase 3 production." @default.
- W4238465239 created "2022-05-12" @default.
- W4238465239 creator A5012386289 @default.
- W4238465239 creator A5032714677 @default.
- W4238465239 creator A5049484320 @default.
- W4238465239 creator A5051185602 @default.
- W4238465239 creator A5053214598 @default.
- W4238465239 creator A5065973541 @default.
- W4238465239 date "2000-09-01" @default.
- W4238465239 modified "2023-10-16" @default.
- W4238465239 title "Modulation of collagenase 3 in human osteoarthritic cartilage by activation of extracellular transforming growth factor β: Role of furin convertase" @default.
- W4238465239 doi "https://doi.org/10.1002/1529-0131(200009)43:9<2100::aid-anr22>3.0.co;2-" @default.
- W4238465239 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11014362" @default.
- W4238465239 hasPublicationYear "2000" @default.
- W4238465239 type Work @default.
- W4238465239 citedByCount "28" @default.
- W4238465239 countsByYear W42384652392012 @default.
- W4238465239 countsByYear W42384652392013 @default.
- W4238465239 countsByYear W42384652392014 @default.
- W4238465239 countsByYear W42384652392017 @default.
- W4238465239 countsByYear W42384652392018 @default.
- W4238465239 countsByYear W42384652392019 @default.
- W4238465239 countsByYear W42384652392021 @default.
- W4238465239 crossrefType "journal-article" @default.
- W4238465239 hasAuthorship W4238465239A5012386289 @default.
- W4238465239 hasAuthorship W4238465239A5032714677 @default.
- W4238465239 hasAuthorship W4238465239A5049484320 @default.
- W4238465239 hasAuthorship W4238465239A5051185602 @default.
- W4238465239 hasAuthorship W4238465239A5053214598 @default.
- W4238465239 hasAuthorship W4238465239A5065973541 @default.
- W4238465239 hasConcept C105702510 @default.
- W4238465239 hasConcept C118131993 @default.
- W4238465239 hasConcept C126322002 @default.
- W4238465239 hasConcept C134018914 @default.
- W4238465239 hasConcept C153911025 @default.
- W4238465239 hasConcept C181199279 @default.
- W4238465239 hasConcept C185592680 @default.
- W4238465239 hasConcept C2777265534 @default.
- W4238465239 hasConcept C2778163477 @default.
- W4238465239 hasConcept C2778229498 @default.
- W4238465239 hasConcept C2778394429 @default.
- W4238465239 hasConcept C2778824825 @default.
- W4238465239 hasConcept C2780072125 @default.
- W4238465239 hasConcept C2780550940 @default.
- W4238465239 hasConcept C2781071845 @default.
- W4238465239 hasConcept C43554185 @default.
- W4238465239 hasConcept C55493867 @default.
- W4238465239 hasConcept C71924100 @default.
- W4238465239 hasConcept C86803240 @default.
- W4238465239 hasConceptScore W4238465239C105702510 @default.
- W4238465239 hasConceptScore W4238465239C118131993 @default.
- W4238465239 hasConceptScore W4238465239C126322002 @default.
- W4238465239 hasConceptScore W4238465239C134018914 @default.
- W4238465239 hasConceptScore W4238465239C153911025 @default.
- W4238465239 hasConceptScore W4238465239C181199279 @default.
- W4238465239 hasConceptScore W4238465239C185592680 @default.
- W4238465239 hasConceptScore W4238465239C2777265534 @default.
- W4238465239 hasConceptScore W4238465239C2778163477 @default.
- W4238465239 hasConceptScore W4238465239C2778229498 @default.
- W4238465239 hasConceptScore W4238465239C2778394429 @default.
- W4238465239 hasConceptScore W4238465239C2778824825 @default.
- W4238465239 hasConceptScore W4238465239C2780072125 @default.
- W4238465239 hasConceptScore W4238465239C2780550940 @default.
- W4238465239 hasConceptScore W4238465239C2781071845 @default.
- W4238465239 hasConceptScore W4238465239C43554185 @default.
- W4238465239 hasConceptScore W4238465239C55493867 @default.
- W4238465239 hasConceptScore W4238465239C71924100 @default.
- W4238465239 hasConceptScore W4238465239C86803240 @default.
- W4238465239 hasIssue "9" @default.
- W4238465239 hasLocation W42384652391 @default.
- W4238465239 hasLocation W42384652392 @default.
- W4238465239 hasOpenAccess W4238465239 @default.
- W4238465239 hasPrimaryLocation W42384652391 @default.
- W4238465239 hasRelatedWork W1491263182 @default.
- W4238465239 hasRelatedWork W2009848449 @default.
- W4238465239 hasRelatedWork W2044094930 @default.
- W4238465239 hasRelatedWork W2064836726 @default.
- W4238465239 hasRelatedWork W2089557302 @default.
- W4238465239 hasRelatedWork W2106604266 @default.
- W4238465239 hasRelatedWork W2155585839 @default.
- W4238465239 hasRelatedWork W2950603898 @default.
- W4238465239 hasRelatedWork W4238465239 @default.
- W4238465239 hasRelatedWork W4322698359 @default.
- W4238465239 hasVolume "43" @default.
- W4238465239 isParatext "false" @default.
- W4238465239 isRetracted "false" @default.
- W4238465239 workType "article" @default.