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- W4238601659 abstract "The enzyme cytosine deaminase (CD) encoded by codA catalyzes deamination of cytosine to uracil. CD is present in prokaryotes and in many eukaryotic micro‐organisms, but is absent in higher plants. 5‐fluorocytosine (5FC) is metabolized in CD‐expressing cells, causing cellular death. A chimeric codA has been introduced into the tobacco plastid genome and 5FC was used to select against tissue culture cells and seedlings expressing CD. This negative selection scheme will be useful in identifying nuclear genes which control plastid gene expression in higher plants." @default.
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- W4238601659 date "1997-09-01" @default.
- W4238601659 modified "2023-09-27" @default.
- W4238601659 title "A negative selection scheme based on the expression of cytosine deaminase in plastids" @default.
- W4238601659 doi "https://doi.org/10.1046/j.1365-313x.1997.d01-17.x" @default.
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