Matches in SemOpenAlex for { <https://semopenalex.org/work/W4239458874> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W4239458874 endingPage "531a" @default.
- W4239458874 startingPage "531a" @default.
- W4239458874 abstract "Kinetochores are macromolecular machines that couple chromosomes to dynamic microtubule tips during cell division, thereby generating force to segregate the chromosomes. Accurate segregation depends on selective stabilization of correct ‘bi-oriented’ kinetochore-microtubule attachments, which come under tension due to opposing forces exerted by microtubules. Tension is thought to stabilize these bi-oriented attachments indirectly, by suppressing the destabilizing activity of a kinase, Aurora B. However, a complete mechanistic understanding of the role of tension requires reconstitution of kinetochore-microtubule attachments for biochemical and biophysical analyses in vitro. Here we show that native kinetochore particles retaining the majority of kinetochore proteins can be purified from budding yeast and used to reconstitute dynamic microtubule attachments. Individual kinetochore particles maintain load-bearing associations with assembling and disassembling ends of single microtubules for >30 min, providing a close match to the persistent coupling seen in vivo between budding yeast kinetochores and single microtubules. Moreover, tension increases the lifetimes of the reconstituted attachments directly, via a catch bond-like mechanismthat does not require Aurora B. Based on these findings, we propose that tension selectively stabilizes proper kinetochore-microtubule attachments in vivo through a combination of direct mechanical stabilization and tension-dependent phosphoregulation." @default.
- W4239458874 created "2022-05-12" @default.
- W4239458874 creator A5005300159 @default.
- W4239458874 creator A5010162864 @default.
- W4239458874 creator A5013591755 @default.
- W4239458874 creator A5020372858 @default.
- W4239458874 creator A5031038205 @default.
- W4239458874 creator A5038047977 @default.
- W4239458874 creator A5074569982 @default.
- W4239458874 creator A5084200031 @default.
- W4239458874 creator A5087456716 @default.
- W4239458874 date "2011-02-01" @default.
- W4239458874 modified "2023-10-16" @default.
- W4239458874 title "Tension Directly Stabilizes Reconstituted Kinetochore-Microtubule Attachments" @default.
- W4239458874 doi "https://doi.org/10.1016/j.bpj.2010.12.3099" @default.
- W4239458874 hasPublicationYear "2011" @default.
- W4239458874 type Work @default.
- W4239458874 citedByCount "0" @default.
- W4239458874 crossrefType "journal-article" @default.
- W4239458874 hasAuthorship W4239458874A5005300159 @default.
- W4239458874 hasAuthorship W4239458874A5010162864 @default.
- W4239458874 hasAuthorship W4239458874A5013591755 @default.
- W4239458874 hasAuthorship W4239458874A5020372858 @default.
- W4239458874 hasAuthorship W4239458874A5031038205 @default.
- W4239458874 hasAuthorship W4239458874A5038047977 @default.
- W4239458874 hasAuthorship W4239458874A5074569982 @default.
- W4239458874 hasAuthorship W4239458874A5084200031 @default.
- W4239458874 hasAuthorship W4239458874A5087456716 @default.
- W4239458874 hasBestOaLocation W42394588741 @default.
- W4239458874 hasConcept C104317684 @default.
- W4239458874 hasConcept C121332964 @default.
- W4239458874 hasConcept C12554922 @default.
- W4239458874 hasConcept C141937037 @default.
- W4239458874 hasConcept C1491633281 @default.
- W4239458874 hasConcept C179254644 @default.
- W4239458874 hasConcept C185592680 @default.
- W4239458874 hasConcept C186068551 @default.
- W4239458874 hasConcept C192838845 @default.
- W4239458874 hasConcept C20418707 @default.
- W4239458874 hasConcept C2778354886 @default.
- W4239458874 hasConcept C30481170 @default.
- W4239458874 hasConcept C47432576 @default.
- W4239458874 hasConcept C54355233 @default.
- W4239458874 hasConcept C74650414 @default.
- W4239458874 hasConcept C85813293 @default.
- W4239458874 hasConcept C86803240 @default.
- W4239458874 hasConcept C93304396 @default.
- W4239458874 hasConcept C95444343 @default.
- W4239458874 hasConcept C9565192 @default.
- W4239458874 hasConceptScore W4239458874C104317684 @default.
- W4239458874 hasConceptScore W4239458874C121332964 @default.
- W4239458874 hasConceptScore W4239458874C12554922 @default.
- W4239458874 hasConceptScore W4239458874C141937037 @default.
- W4239458874 hasConceptScore W4239458874C1491633281 @default.
- W4239458874 hasConceptScore W4239458874C179254644 @default.
- W4239458874 hasConceptScore W4239458874C185592680 @default.
- W4239458874 hasConceptScore W4239458874C186068551 @default.
- W4239458874 hasConceptScore W4239458874C192838845 @default.
- W4239458874 hasConceptScore W4239458874C20418707 @default.
- W4239458874 hasConceptScore W4239458874C2778354886 @default.
- W4239458874 hasConceptScore W4239458874C30481170 @default.
- W4239458874 hasConceptScore W4239458874C47432576 @default.
- W4239458874 hasConceptScore W4239458874C54355233 @default.
- W4239458874 hasConceptScore W4239458874C74650414 @default.
- W4239458874 hasConceptScore W4239458874C85813293 @default.
- W4239458874 hasConceptScore W4239458874C86803240 @default.
- W4239458874 hasConceptScore W4239458874C93304396 @default.
- W4239458874 hasConceptScore W4239458874C95444343 @default.
- W4239458874 hasConceptScore W4239458874C9565192 @default.
- W4239458874 hasIssue "3" @default.
- W4239458874 hasLocation W42394588741 @default.
- W4239458874 hasLocation W42394588742 @default.
- W4239458874 hasOpenAccess W4239458874 @default.
- W4239458874 hasPrimaryLocation W42394588741 @default.
- W4239458874 hasRelatedWork W1991732228 @default.
- W4239458874 hasRelatedWork W2000164138 @default.
- W4239458874 hasRelatedWork W2022565225 @default.
- W4239458874 hasRelatedWork W2069200157 @default.
- W4239458874 hasRelatedWork W2106148947 @default.
- W4239458874 hasRelatedWork W2132061062 @default.
- W4239458874 hasRelatedWork W2157475559 @default.
- W4239458874 hasRelatedWork W2168908838 @default.
- W4239458874 hasRelatedWork W2991245854 @default.
- W4239458874 hasRelatedWork W4285045694 @default.
- W4239458874 hasVolume "100" @default.
- W4239458874 isParatext "false" @default.
- W4239458874 isRetracted "false" @default.
- W4239458874 workType "article" @default.