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- W4239485256 abstract "Background. The optimal use of blood cultures to determine the etiology of febrile episodes in neutropenic children has not been well-defined. Methods. Single volume blood cultures using the Pediatric ISOLATOR System (ISO), were compared with variable, weight-based culture volumes using the BACTEC 9240 Culture System (BAC). Additionally the value of routinely inoculating the BACTEC MYCO/F LYTIC culture vial (MFL) as well as the BACTEC AEROBIC/F culture vial (AF) was examined Results. A total of 2620 cultures had both ISO and BAC inoculated; 182 cultures were positive (7.0% of cultures); 97.8% of positive cultures were detected by the BAC (AF and/or MFL) vs. 46.2% detected by the ISO. The advantage of the BAC over the ISO was statistically significant for overall recovery of isolates and bloodstream infections, including most individual organism categories. There were only two instances (one each of histoplasmosis and candidemia) in which a blood stream infection was detected by ISO only. All the isolates judged to be contaminants were recovered by BAC only. AF detected significantly more coagulase-negative Staphylococcus spp. than the MFL. Of the isolates 16%, representing 14% of the bloodstream infections (including Gram-negative infections), were detected by the MFL only. Infections were detected more quickly by BAC than by ISO (P < 0.0001). Among the BAC media types, AF was faster than MFL (P < 0.0001). Conclusions. Optimal yield of blood cultures in immunocompromised pediatric patients included the use of BAC with a weight-based, graduated volume of culture inoculation and routine use of both AF and MFL." @default.
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- W4239485256 date "2003-06-01" @default.
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- W4239485256 doi "https://doi.org/10.1097/00006454-200306000-00011" @default.
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