Matches in SemOpenAlex for { <https://semopenalex.org/work/W4239789214> ?p ?o ?g. }
- W4239789214 endingPage "10908" @default.
- W4239789214 startingPage "10904" @default.
- W4239789214 abstract "Abstract Nuclear magnetic resonance (NMR) spectroscopy has the intrinsic capabilities to investigate proteins in native environments. In general, however, NMR relies on non‐natural protein purity and concentration to increase the desired signal over the background. We here report on the efficient and specific hyperpolarization of low amounts of a target protein in a large isotope‐labeled background by combining dynamic nuclear polarization (DNP) and the selectivity of protein interactions. Using a biradical‐labeled ligand, we were able to direct the hyperpolarization to the protein of interest, maintaining comparable signal enhancement with about 400‐fold less radicals than conventionally used. We could selectively filter out our target protein directly from crude cell lysate obtained from only 8 mL of fully isotope‐enriched cell culture. Our approach offers effective means to study proteins with atomic resolution in increasingly native concentrations and environments." @default.
- W4239789214 created "2022-05-12" @default.
- W4239789214 creator A5016715135 @default.
- W4239789214 creator A5034359184 @default.
- W4239789214 creator A5055646251 @default.
- W4239789214 creator A5060774311 @default.
- W4239789214 creator A5062917568 @default.
- W4239789214 creator A5072263385 @default.
- W4239789214 creator A5075772816 @default.
- W4239789214 creator A5076636733 @default.
- W4239789214 creator A5078752390 @default.
- W4239789214 date "2016-06-28" @default.
- W4239789214 modified "2023-10-18" @default.
- W4239789214 title "Selective Protein Hyperpolarization in Cell Lysates Using Targeted Dynamic Nuclear Polarization" @default.
- W4239789214 cites W1599375256 @default.
- W4239789214 cites W1601014792 @default.
- W4239789214 cites W1780526636 @default.
- W4239789214 cites W1947808677 @default.
- W4239789214 cites W1971283335 @default.
- W4239789214 cites W1972729603 @default.
- W4239789214 cites W2020536166 @default.
- W4239789214 cites W2022252221 @default.
- W4239789214 cites W2057547892 @default.
- W4239789214 cites W2065265087 @default.
- W4239789214 cites W207739447 @default.
- W4239789214 cites W2089914156 @default.
- W4239789214 cites W2091113012 @default.
- W4239789214 cites W2106799894 @default.
- W4239789214 cites W2108589844 @default.
- W4239789214 cites W2108846485 @default.
- W4239789214 cites W2111777928 @default.
- W4239789214 cites W2121473506 @default.
- W4239789214 cites W2135060180 @default.
- W4239789214 cites W2144176808 @default.
- W4239789214 cites W2145873333 @default.
- W4239789214 cites W2151953994 @default.
- W4239789214 cites W2169388859 @default.
- W4239789214 cites W2170823043 @default.
- W4239789214 cites W2253387945 @default.
- W4239789214 cites W2326876960 @default.
- W4239789214 cites W2419385889 @default.
- W4239789214 cites W2444601377 @default.
- W4239789214 cites W4242119823 @default.
- W4239789214 cites W4245448735 @default.
- W4239789214 doi "https://doi.org/10.1002/ange.201603205" @default.
- W4239789214 hasPublicationYear "2016" @default.
- W4239789214 type Work @default.
- W4239789214 citedByCount "19" @default.
- W4239789214 countsByYear W42397892142017 @default.
- W4239789214 countsByYear W42397892142018 @default.
- W4239789214 countsByYear W42397892142019 @default.
- W4239789214 countsByYear W42397892142020 @default.
- W4239789214 countsByYear W42397892142021 @default.
- W4239789214 crossrefType "journal-article" @default.
- W4239789214 hasAuthorship W4239789214A5016715135 @default.
- W4239789214 hasAuthorship W4239789214A5034359184 @default.
- W4239789214 hasAuthorship W4239789214A5055646251 @default.
- W4239789214 hasAuthorship W4239789214A5060774311 @default.
- W4239789214 hasAuthorship W4239789214A5062917568 @default.
- W4239789214 hasAuthorship W4239789214A5072263385 @default.
- W4239789214 hasAuthorship W4239789214A5075772816 @default.
- W4239789214 hasAuthorship W4239789214A5076636733 @default.
- W4239789214 hasAuthorship W4239789214A5078752390 @default.
- W4239789214 hasConcept C121332964 @default.
- W4239789214 hasConcept C12554922 @default.
- W4239789214 hasConcept C131453863 @default.
- W4239789214 hasConcept C147789679 @default.
- W4239789214 hasConcept C185592680 @default.
- W4239789214 hasConcept C205049153 @default.
- W4239789214 hasConcept C46141821 @default.
- W4239789214 hasConcept C55493867 @default.
- W4239789214 hasConcept C57409179 @default.
- W4239789214 hasConcept C66974803 @default.
- W4239789214 hasConcept C71240020 @default.
- W4239789214 hasConcept C86803240 @default.
- W4239789214 hasConceptScore W4239789214C121332964 @default.
- W4239789214 hasConceptScore W4239789214C12554922 @default.
- W4239789214 hasConceptScore W4239789214C131453863 @default.
- W4239789214 hasConceptScore W4239789214C147789679 @default.
- W4239789214 hasConceptScore W4239789214C185592680 @default.
- W4239789214 hasConceptScore W4239789214C205049153 @default.
- W4239789214 hasConceptScore W4239789214C46141821 @default.
- W4239789214 hasConceptScore W4239789214C55493867 @default.
- W4239789214 hasConceptScore W4239789214C57409179 @default.
- W4239789214 hasConceptScore W4239789214C66974803 @default.
- W4239789214 hasConceptScore W4239789214C71240020 @default.
- W4239789214 hasConceptScore W4239789214C86803240 @default.
- W4239789214 hasFunder F4320311647 @default.
- W4239789214 hasFunder F4320332735 @default.
- W4239789214 hasIssue "36" @default.
- W4239789214 hasLocation W42397892141 @default.
- W4239789214 hasOpenAccess W4239789214 @default.
- W4239789214 hasPrimaryLocation W42397892141 @default.
- W4239789214 hasRelatedWork W1551020276 @default.
- W4239789214 hasRelatedWork W2035075384 @default.
- W4239789214 hasRelatedWork W2145880044 @default.
- W4239789214 hasRelatedWork W2292146963 @default.
- W4239789214 hasRelatedWork W2359497114 @default.