Matches in SemOpenAlex for { <https://semopenalex.org/work/W4240003114> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W4240003114 endingPage "260" @default.
- W4240003114 startingPage "256" @default.
- W4240003114 abstract "The present experiments were undertaken to investigate whether the procedure of intracytoplasmic sperm injection (ICSI) is associated with changes in the intracellular free calcium concentration ([Ca2+]i). [Ca2+]i was measured, using the calcium-sensitive dye fura-2, during and after impalement of mouse oocytes with an ICSI pipette and injection of a small amount of medium alone or of medium containing a normal human spermatozoon. Forty-five oocytes were injected with medium. Two different responses were observed: 20 of these cells showed a large increase of [Ca2+]i upon impalement; the other 25 cells did not show any change of [Ca2+]i, neither in the acute period nor in a late period 4 hr after impalement. All the cells that responded with an increase of [Ca2+]i subsequently lysed within the first 30 min following impalement, while all the cells with no [Ca2+]i change remained intact. This observation suggests that only traumatic impalement is associated with an increase of [Ca2+]i. Thirty-one oocytes were successfully, i.e., without subsequent cell lysis, injected with a normal mouse or human spermatozoon. In none of these cells could any acute or late change of [Ca2+]i be observed. The experiments illustrate that successful performance of the ICSI procedure, i.e., ICSI not followed by cell lysis, is not associated with changes of [Ca2+]i in mouse oocytes. This suggests that the ICSI technique, by itself, does not help in activating the oocyte via manipulation-induced changes of [Ca2+]i. © 1996 Wiley-Liss, Inc." @default.
- W4240003114 created "2022-05-12" @default.
- W4240003114 creator A5049842457 @default.
- W4240003114 creator A5055601210 @default.
- W4240003114 creator A5065437445 @default.
- W4240003114 creator A5086097021 @default.
- W4240003114 date "1996-02-01" @default.
- W4240003114 modified "2023-09-25" @default.
- W4240003114 title "Changes of intracellular free calcium during intracytoplasmic sperm injection" @default.
- W4240003114 doi "https://doi.org/10.1002/(sici)1098-2795(199602)43:2<256::aid-mrd15>3.3.co;2-b" @default.
- W4240003114 hasPublicationYear "1996" @default.
- W4240003114 type Work @default.
- W4240003114 citedByCount "0" @default.
- W4240003114 crossrefType "journal-article" @default.
- W4240003114 hasAuthorship W4240003114A5049842457 @default.
- W4240003114 hasAuthorship W4240003114A5055601210 @default.
- W4240003114 hasAuthorship W4240003114A5065437445 @default.
- W4240003114 hasAuthorship W4240003114A5086097021 @default.
- W4240003114 hasConcept C105702510 @default.
- W4240003114 hasConcept C126322002 @default.
- W4240003114 hasConcept C147789679 @default.
- W4240003114 hasConcept C151552171 @default.
- W4240003114 hasConcept C153911025 @default.
- W4240003114 hasConcept C16685009 @default.
- W4240003114 hasConcept C179265757 @default.
- W4240003114 hasConcept C181080969 @default.
- W4240003114 hasConcept C185592680 @default.
- W4240003114 hasConcept C196843134 @default.
- W4240003114 hasConcept C2776632385 @default.
- W4240003114 hasConcept C2776690073 @default.
- W4240003114 hasConcept C2778278907 @default.
- W4240003114 hasConcept C2781087480 @default.
- W4240003114 hasConcept C512716672 @default.
- W4240003114 hasConcept C519063684 @default.
- W4240003114 hasConcept C57409179 @default.
- W4240003114 hasConcept C62525196 @default.
- W4240003114 hasConcept C71924100 @default.
- W4240003114 hasConcept C79879829 @default.
- W4240003114 hasConcept C86803240 @default.
- W4240003114 hasConcept C88972607 @default.
- W4240003114 hasConcept C95444343 @default.
- W4240003114 hasConceptScore W4240003114C105702510 @default.
- W4240003114 hasConceptScore W4240003114C126322002 @default.
- W4240003114 hasConceptScore W4240003114C147789679 @default.
- W4240003114 hasConceptScore W4240003114C151552171 @default.
- W4240003114 hasConceptScore W4240003114C153911025 @default.
- W4240003114 hasConceptScore W4240003114C16685009 @default.
- W4240003114 hasConceptScore W4240003114C179265757 @default.
- W4240003114 hasConceptScore W4240003114C181080969 @default.
- W4240003114 hasConceptScore W4240003114C185592680 @default.
- W4240003114 hasConceptScore W4240003114C196843134 @default.
- W4240003114 hasConceptScore W4240003114C2776632385 @default.
- W4240003114 hasConceptScore W4240003114C2776690073 @default.
- W4240003114 hasConceptScore W4240003114C2778278907 @default.
- W4240003114 hasConceptScore W4240003114C2781087480 @default.
- W4240003114 hasConceptScore W4240003114C512716672 @default.
- W4240003114 hasConceptScore W4240003114C519063684 @default.
- W4240003114 hasConceptScore W4240003114C57409179 @default.
- W4240003114 hasConceptScore W4240003114C62525196 @default.
- W4240003114 hasConceptScore W4240003114C71924100 @default.
- W4240003114 hasConceptScore W4240003114C79879829 @default.
- W4240003114 hasConceptScore W4240003114C86803240 @default.
- W4240003114 hasConceptScore W4240003114C88972607 @default.
- W4240003114 hasConceptScore W4240003114C95444343 @default.
- W4240003114 hasIssue "2" @default.
- W4240003114 hasLocation W42400031141 @default.
- W4240003114 hasOpenAccess W4240003114 @default.
- W4240003114 hasPrimaryLocation W42400031141 @default.
- W4240003114 hasRelatedWork W1909590713 @default.
- W4240003114 hasRelatedWork W2022065753 @default.
- W4240003114 hasRelatedWork W2037533250 @default.
- W4240003114 hasRelatedWork W2045299582 @default.
- W4240003114 hasRelatedWork W2107771556 @default.
- W4240003114 hasRelatedWork W2136200022 @default.
- W4240003114 hasRelatedWork W2155020902 @default.
- W4240003114 hasRelatedWork W2302765798 @default.
- W4240003114 hasRelatedWork W2374381209 @default.
- W4240003114 hasRelatedWork W2166577723 @default.
- W4240003114 hasVolume "43" @default.
- W4240003114 isParatext "false" @default.
- W4240003114 isRetracted "false" @default.
- W4240003114 workType "article" @default.