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- W4240032013 abstract "A sensitive kinetic determination of serum 5′-nucleotidase activity is described. Nucleoside oxidase catalyzes the oxidative coupling reaction of phenolic compounds and 4-aminoantipyrine to form colored substances in proportion of the amount of nucleoside. The present method relies on this phenomenon. Human serum 5′-nucleotidase was assayed using 5′-AMP as a substrate. When adenosine liberated was oxidized by nucleoside oxidase in the presence of phenolic compounds and 4-aminoantipyrine, a colored substance was formed at the same time. The rate of colored substance formation was proportional to the 5′-nucleotidase activity. The proposed method shows a low variation and a good correlation with the reference method. 3′-Nucleotidase was also assayed in a similar manner." @default.
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- W4240032013 date "1989-10-01" @default.
- W4240032013 modified "2023-10-16" @default.
- W4240032013 title "A New Kinetic Method for Assaying Serum 5′-Nucleotidase Using Nucleoside Oxidase" @default.
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- W4240032013 doi "https://doi.org/10.1080/00021369.1989.10869698" @default.
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