FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4240171806> ?p ?o ?g. }

• W4240171806 abstract "Abstract Background Unlike mammals, goose fatty liver shows a strong tolerance to fatty acids without obvious injury. Stearyl-coenzyme A desaturase 1 (SCD1) serves crucial role in desaturation of saturated fatty acids (SAFs), but its role in the SAFs tolerance of goose hepatocytes has not been reported. This study was conducted to explore the role of SCD1 in regulating palmitic acid tolerance of goose primary hepatocytes. Methods To evaluate the palmitic acid tolerance of cultured hepatocytes, MTT was examined to reflect the effect of palmitic acid on cell viability, and quantitative PCR was used to detect the mRNA expression levels of several genes related to ER stress, inflammation, and apoptosis, and the role of SCD1 in palmitic acid tolerance of goose hepatocytes was explored using RNA interfere. Results Our results indicated that goose hepatocytes exhibited a higher tolerant capacity to palmitic acid than human hepatic cell line (LO2 cells). Furthermore, the mRNA levels of fatty acid desaturation-related genes ( SCD1 and FADS2 ) and fatty acid elongate enzyme-related gene ( ELOVL6 ) were significantly upregulated in goose primary hepatocytes treated with 0.6 mM palmitic acid. However, in cultured LO2 cells, expression of ER stress-related genes ( XBP , BIP and ATF6 ), inflammatory response-related genes ( IL-6 , IL-1β and IFN-γ ) and apoptosis-related genes ( Bax , Bcl-2 , Caspase-3 and Caspase-9 ) was significantly enhanced by the addition of 0.6 mM palmitic acid. Additionally, siRNA-mediated downregulation of SCD1 significantly reduced the palmitic acid tolerance of goose primary hepatocytes under the treatment of 0.6 mM palmitic acid; meanwhile, the mRNA expression of inflammatory-related genes ( IL-6 and IL-1β ) and several key genes involved in the PI3K/AKT, FoxO1, mTOR and AMPK pathways ( AKT1 , AKT2 , FOXO1 and SIRT1 ), as well as the protein expression of cytochrome C and the apoptosis rate were also upregulated. Conclusion In conclusion, our data suggested that SCD1 is involved in enhancing the palmitic acid tolerance of goose primary hepatocytes by regulating inflammation- and apoptosis-related genes expression." @default.
• W4240171806 created "2022-05-12" @default.
• W4240171806 creator A5000570295 @default.
• W4240171806 creator A5028449554 @default.
• W4240171806 creator A5033782526 @default.
• W4240171806 creator A5064564309 @default.
• W4240171806 creator A5075840318 @default.
• W4240171806 date "2020-03-23" @default.
• W4240171806 modified "2023-09-30" @default.
• W4240171806 title "Role of the stearyl-coenzyme A desaturase 1 gene in regulating palmitic acid tolerance of goose primary hepatocytes" @default.
• W4240171806 doi "https://doi.org/10.21203/rs.3.rs-18680/v1" @default.
• W4240171806 hasPublicationYear "2020" @default.
• W4240171806 type Work @default.
• W4240171806 citedByCount "0" @default.
• W4240171806 crossrefType "posted-content" @default.
• W4240171806 hasAuthorship W4240171806A5000570295 @default.
• W4240171806 hasAuthorship W4240171806A5028449554 @default.
• W4240171806 hasAuthorship W4240171806A5033782526 @default.
• W4240171806 hasAuthorship W4240171806A5064564309 @default.
• W4240171806 hasAuthorship W4240171806A5075840318 @default.
• W4240171806 hasBestOaLocation W42401718061 @default.
• W4240171806 hasConcept C151730666 @default.
• W4240171806 hasConcept C153911025 @default.
• W4240171806 hasConcept C185592680 @default.
• W4240171806 hasConcept C2779936771 @default.
• W4240171806 hasConcept C2781126499 @default.
• W4240171806 hasConcept C543025807 @default.
• W4240171806 hasConcept C55493867 @default.
• W4240171806 hasConcept C86803240 @default.
• W4240171806 hasConceptScore W4240171806C151730666 @default.
• W4240171806 hasConceptScore W4240171806C153911025 @default.
• W4240171806 hasConceptScore W4240171806C185592680 @default.
• W4240171806 hasConceptScore W4240171806C2779936771 @default.
• W4240171806 hasConceptScore W4240171806C2781126499 @default.
• W4240171806 hasConceptScore W4240171806C543025807 @default.
• W4240171806 hasConceptScore W4240171806C55493867 @default.
• W4240171806 hasConceptScore W4240171806C86803240 @default.
• W4240171806 hasLocation W42401718061 @default.
• W4240171806 hasOpenAccess W4240171806 @default.
• W4240171806 hasPrimaryLocation W42401718061 @default.
• W4240171806 hasRelatedWork W13810224 @default.
• W4240171806 hasRelatedWork W16006477 @default.
• W4240171806 hasRelatedWork W16698073 @default.
• W4240171806 hasRelatedWork W1937665 @default.
• W4240171806 hasRelatedWork W19804102 @default.
• W4240171806 hasRelatedWork W20243291 @default.
• W4240171806 hasRelatedWork W20991648 @default.
• W4240171806 hasRelatedWork W4464528 @default.
• W4240171806 hasRelatedWork W4714812 @default.
• W4240171806 hasRelatedWork W8048107 @default.
• W4240171806 isParatext "false" @default.
• W4240171806 isRetracted "false" @default.
• W4240171806 workType "article" @default.