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• W4240219052 abstract "Isolation of cells from heterogeneous biological samples is critical in both basic biological research and clinical diagnostics. Affinity-based methods, such as those that recognise cells by binding antibodies to cell membrane biomarkers, can be used to achieve specific cell isolation. Microfluidic techniques have been employed to achieve more efficient and effective cell isolation. By employing aptamers as surface-immobilised ligands, cells can be easily released and collected after specific capture. However, these methods still have limitations in cell release efficiency and spatial selectivity. This study presents an aptamer-based microfluidic device that not only achieves specific affinity cell capture, but also enables spatially selective temperature-mediated release and retrieval of cells without detectable damage. The specific cell capture is realised by using surface-patterned aptamers in a microchamber on a temperature-control chip. Spatially selective cell release is achieved by utilising a group of microheater and temperature sensor that restricts temperature changes, and therefore the disruption of cell-aptamer interactions, to a design-specified region. Experimental results with CCRF-CEM cells and sgc8c aptamers have demonstrated the specific cell capture and temperature-mediated release of selected groups of cells with negligible disruption to their viability." @default.
• W4240219052 created "2022-05-12" @default.
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• W4240219052 date "2014-03-01" @default.
• W4240219052 modified "2023-10-18" @default.
• W4240219052 title "Spatially selective release of aptamer‐captured cells by temperature mediation" @default.
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• W4240219052 doi "https://doi.org/10.1049/iet-nbt.2013.0028" @default.
• W4240219052 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24888185" @default.
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