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• W4240400188 endingPage "679" @default.
• W4240400188 startingPage "673" @default.
• W4240400188 abstract "The cytoplasm of Saccharomyces cerevisiae contains two major classes of protein-encapsulated double-stranded ribonucleic acids (dsRNA's), L and M. Replication of L and M dsRNA's was examined in cells arrested in the G1 phase by either alpha-factor, a yeast mating pheromone, or the restrictive temperature for a cell cycle mutant (cdc7). [3H]uracil was added during the arrest periods to cells prelabeled with [14C]uracil, and replication was monitored by determining the ratio of 3H/14C for purified dsRNA's. Like mitochondrial deoxyribonucleic acid, both L and M dsRNA's were synthesized in the G1 arrested cells. The replication of L dsRNA was also examined during the S phase, using cells synchronized in two different ways. Cells containing the cdc7 mutation, treated sequentially with alpha-factor and then the restrictive temperature, enter a synchronous S phase when transferred to permissive temperature. When cells entered the S phase, synthesis of L dsRNA ceased, and little or no synthesis was detected throughout the S phase. Synthesis of L dsRNA was also observed in G1 phase cells isolated from asynchronous cultures by velocity centrifugation. Again, synthesis ceased when cells entered the S phase. These results indicate that L dsRNA replication is under cell cycle control. The control differs from that of mitochondrial deoxyribonucleic acid, which replicates in all phases of the cell cycle, and from that of 2-micron DNA, a multiple-copy plasmid whose replication is confined to the S phase." @default.
• W4240400188 created "2022-05-12" @default.
• W4240400188 creator A5006524357 @default.
• W4240400188 creator A5037017638 @default.
• W4240400188 creator A5069731363 @default.
• W4240400188 date "1981-08-01" @default.
• W4240400188 modified "2023-10-03" @default.
• W4240400188 title "Yeast L double-stranded ribonucleic acid is synthesized during the G1 phase but not the S phase of the cell cycle" @default.
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• W4240400188 doi "https://doi.org/10.1128/mcb.1.8.673-679.1981" @default.
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