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- W4240618265 abstract "A number of challenges have emerged in the discussion of super-resolution microscopy techniques. These can be divided into application-oriented challenges and physicochemical challenges, though ultimately overcoming the latter will also help in tackling the former. For many studies, a correlation of super-resolution microscopy images with those obtained by electron microscopy (EM) with its even higher resolution represents another important step forward. Abandoning the high illumination power requirements of present stimulated emission depletion (STED) microscopy and single-molecule localization microscopy (SMLM) methods constitutes a major advancement. SMLM imaging based on color separation offers the possibility to obtain fluorescence images with linear excitation schemes. A first major breakthrough in the application of super-resolution microscopy was achieved by the commercialization of such microscope devices. A second technical breakthrough might be expected from the commercial implementation of 3D high-precision z-resolution. Enhanced illumination modes and novel dyes and labeling techniques are likely to be the key for routine in vivo imaging using super-resolution microscopy." @default.
- W4240618265 created "2022-05-12" @default.
- W4240618265 date "2017-08-25" @default.
- W4240618265 modified "2023-10-17" @default.
- W4240618265 title "Discussion" @default.
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- W4240618265 doi "https://doi.org/10.1002/9783527802074.ch8" @default.
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