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- W4240652603 abstract "Aim. Estimation of the allelic status of marker crtRB1-3'TE of the β-carotene hydroxylase gene and marker-assisted selection by this marker in Ukrainian maize breeding material. Methods. Field method and polymerase chain reaction. Results. The analysis of the allelic state of β-carotene hydroxylase gene for marker crtRB1-3'TE in maize breeding populations (DK23×F2)F2 and (DK23×F2)F3MAS having been obtained after the first and second self-pollinations of single cross DK23×F2 was provided. It was established that the parental inbred lines DK23 and F2 contained respectively 296 bp (unfavorable) and 543 bp (favorable) alleles of marker crtRB1-3'TE. The three kinds of genotypes appeared to present at different frequencies in (DK23×F2)F2 – homozygous for allele 296 bp, homozygous for allele 543 bp and heterozygous with both alleles 296 bp and 543 bp. For further cultivation and self-pollination, only plants with allele 543 bp within (DK23×F2)F2 were selected. All tested plants in population (DK23×F2)F3MAS were homozygous for allele 543 bp. Conclusions. Marker-associated selection in two generations for the β-carotene hydroxylase gene, involved in β-carotene accumulation, allowed to select homozygous plants of maize by favorable crtRB1-3'TE allele.
 Keywords: Zea mays L., molecular genetic markers, carotenoids, breeding populations, allele." @default.
- W4240652603 created "2022-05-12" @default.
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- W4240652603 date "2020-09-01" @default.
- W4240652603 modified "2023-10-16" @default.
- W4240652603 title "Marker-assisted selection for the gene of β-carotene hydroxylase in maize" @default.
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- W4240652603 doi "https://doi.org/10.7124/feeo.v27.1307" @default.
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