Matches in SemOpenAlex for { <https://semopenalex.org/work/W4240717199> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W4240717199 abstract "Abstract Background: Systemic transplantation of stem cells from human exfoliated deciduous teeth (SHED) recover bone loss in animal models of osteoporosis; however, the mechanisms underlying this remain unclear. Here, we hypothesized that trophic factors within SHED-releasing extracellular vesicles (SHED-EVs) rescue osteoporotic phenotype. Methods: EVs were isolated from culture supernatant of SHED. SHED-EVs were treated with or without ribonuclease and systemically administrated into ovariectomized mice, followed by the function of recipient bone marrow mesenchymal stem cells (BMMSCs) including telomerase activity, osteoblast differentiation, and sepmaphorine-3A (SEMA3A) secretion. Subsequently, human BMMSCs were stimulated by SHED-EVs with or without ribonuclease treatment, and then human BMMSCs were examined the function of telomerase activity, osteoblast differentiation, and SEMA3A secretion. Furthermore, SHED-EVs treated human BMMSCs were subcutaneously transplanted into dorsal skin of immunocompromised mice with hydroxyapatite tricalcium phosphate (HA/TCP) careers and analyzed the de novo bone forming ability. Results: We revealed that systemic SHED-EV-infusion recovered bone volume in ovariectomized mice and improved the function of recipient BMMSCs by rescuing the mRNA levels of Tert and telomerase activity, osteoblast differentiation, and SEMA3A secretion. Ribonuclease treatment depleted RNAs, including microRNAs, within SHED-EVs and these RNA-depleted SHED-EVs attenuated SHED-EV-rescued function of recipient BMMSCs in the ovariectomized mice. These findings were supported by in vitro assays using human BMMSCs incubated with SHED-EVs. Conclusion: Collectively, our findings suggest that SHED-secreted RNAs, such as microRNAs, play a crucial role in treating postmenopausal osteoporosis by targeting the telomerase activity of recipient BMMSCs." @default.
- W4240717199 created "2022-05-12" @default.
- W4240717199 creator A5003879012 @default.
- W4240717199 creator A5004921304 @default.
- W4240717199 creator A5006570400 @default.
- W4240717199 creator A5007441454 @default.
- W4240717199 creator A5026664767 @default.
- W4240717199 creator A5033478195 @default.
- W4240717199 creator A5040763253 @default.
- W4240717199 creator A5043317558 @default.
- W4240717199 creator A5050378903 @default.
- W4240717199 creator A5063186880 @default.
- W4240717199 date "2020-06-30" @default.
- W4240717199 modified "2023-09-29" @default.
- W4240717199 title "Extracellular vesicles from deciduous pulp stem cells recover bone loss by regulating telomerase activity in an osteoporosis mouse model" @default.
- W4240717199 doi "https://doi.org/10.21203/rs.3.rs-20407/v2" @default.
- W4240717199 hasPublicationYear "2020" @default.
- W4240717199 type Work @default.
- W4240717199 citedByCount "0" @default.
- W4240717199 crossrefType "posted-content" @default.
- W4240717199 hasAuthorship W4240717199A5003879012 @default.
- W4240717199 hasAuthorship W4240717199A5004921304 @default.
- W4240717199 hasAuthorship W4240717199A5006570400 @default.
- W4240717199 hasAuthorship W4240717199A5007441454 @default.
- W4240717199 hasAuthorship W4240717199A5026664767 @default.
- W4240717199 hasAuthorship W4240717199A5033478195 @default.
- W4240717199 hasAuthorship W4240717199A5040763253 @default.
- W4240717199 hasAuthorship W4240717199A5043317558 @default.
- W4240717199 hasAuthorship W4240717199A5050378903 @default.
- W4240717199 hasAuthorship W4240717199A5063186880 @default.
- W4240717199 hasBestOaLocation W42407171991 @default.
- W4240717199 hasConcept C134018914 @default.
- W4240717199 hasConcept C198826908 @default.
- W4240717199 hasConcept C202751555 @default.
- W4240717199 hasConcept C203014093 @default.
- W4240717199 hasConcept C2776541429 @default.
- W4240717199 hasConcept C2778260815 @default.
- W4240717199 hasConcept C502942594 @default.
- W4240717199 hasConcept C55493867 @default.
- W4240717199 hasConcept C86803240 @default.
- W4240717199 hasConcept C95444343 @default.
- W4240717199 hasConceptScore W4240717199C134018914 @default.
- W4240717199 hasConceptScore W4240717199C198826908 @default.
- W4240717199 hasConceptScore W4240717199C202751555 @default.
- W4240717199 hasConceptScore W4240717199C203014093 @default.
- W4240717199 hasConceptScore W4240717199C2776541429 @default.
- W4240717199 hasConceptScore W4240717199C2778260815 @default.
- W4240717199 hasConceptScore W4240717199C502942594 @default.
- W4240717199 hasConceptScore W4240717199C55493867 @default.
- W4240717199 hasConceptScore W4240717199C86803240 @default.
- W4240717199 hasConceptScore W4240717199C95444343 @default.
- W4240717199 hasLocation W42407171991 @default.
- W4240717199 hasLocation W42407171992 @default.
- W4240717199 hasLocation W42407171993 @default.
- W4240717199 hasOpenAccess W4240717199 @default.
- W4240717199 hasPrimaryLocation W42407171991 @default.
- W4240717199 hasRelatedWork W16821395 @default.
- W4240717199 hasRelatedWork W2290790 @default.
- W4240717199 hasRelatedWork W2394888 @default.
- W4240717199 hasRelatedWork W31203876 @default.
- W4240717199 hasRelatedWork W31847455 @default.
- W4240717199 hasRelatedWork W32014332 @default.
- W4240717199 hasRelatedWork W36068157 @default.
- W4240717199 hasRelatedWork W38324799 @default.
- W4240717199 hasRelatedWork W38715709 @default.
- W4240717199 hasRelatedWork W9867326 @default.
- W4240717199 isParatext "false" @default.
- W4240717199 isRetracted "false" @default.
- W4240717199 workType "article" @default.